GPR30/GPER-1 mediates rapid decreases in TLR4 expression on murine macrophages

Molecular and Cellular Endocrinology
Jennifer A RettewIan Marriott

Abstract

Studies to define the effects of estrogens on immune function have yielded conflicting results. The recent demonstration that GPR30 can mediate rapid non-genomic events and may function as a novel transmembrane estrogen receptor could provide a mechanism underlying such findings. In this study, we have investigated the ability of GPR30 to regulate cell-surface expression of Toll-like receptor 4 (TLR4), a key molecule in the perception of bacterial lipopolysaccharide (LPS) by immune cells. We show that 17beta-estradiol or GPR30-specific agonists decrease TLR4 expression on macrophages within 10-60 min and such effects were abolished following GPR30 knockdown. Importantly, GPR30 ligation significantly reduces sensitivity of these immune cells to LPS challenge as determined by reductions in inflammatory mediator production. Based on these findings, we suggest that estrogen may utilize this non-classical estrogen receptor to limit potentially lethal acute inflammatory responses without compromising long-term host defense.

References

Nov 20, 1998·Archives of Surgery·J SchröderF Stüber
Apr 12, 2001·Clinical and Experimental Immunology·K TsuyuguchiF Kuze
Oct 1, 1964·The Journal of Endocrinology·T NICOLB VERNON-ROBERTS
Sep 24, 2004·Immunology·Eva M Pålsson-McDermott, Luke A J O'Neill
Dec 29, 2004·Transplantation Proceedings·M SahinS Hengirmen
Aug 24, 2005·The Journal of Surgical Research·Göksel SenerBerrak C Yeğen
Oct 8, 2005·Stroke; a Journal of Cerebral Circulation·Daniel RoyJean Raymond
Jan 18, 2006·Cell Death and Differentiation·T Kawai, S Akira
Mar 8, 2006·Nature Chemical Biology·Cristian G BologaEric R Prossnitz
Oct 3, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Samuel H McCallIan Marriott
Dec 20, 2007·Critical Care Medicine·Addison K MayRobert G Sawyer
Feb 15, 2008·Annual Review of Physiology·Eric R ProssnitzHelen J Hathaway
Mar 1, 2008·Clinical and Experimental Pharmacology & Physiology·P GourdyJ F Arnal
Mar 28, 2008·Surgical Infections·Lesly A DossettAddison K May
May 26, 2009·Molecular and Cellular Endocrinology·Eric R Prossnitz, Marcello Maggiolini
Aug 12, 2009·Journal of Neuroimmunology·Eric BlaskoRichard Horuk
Dec 26, 2009·Steroids·Gernot LangerChristiane Otto

❮ Previous
Next ❯

Citations

Feb 20, 2013·Journal of Assisted Reproduction and Genetics·Yi-Ran LiRi-Cheng Chian
Aug 17, 2011·Nature Reviews. Endocrinology·Eric R Prossnitz, Matthias Barton
Feb 23, 2012·Journal of Molecular Endocrinology·Jerzy-Roch Nofer
Feb 18, 2014·Molecular and Cellular Endocrinology·Eric R Prossnitz, Matthias Barton
Jan 21, 2014·Experimental Neurology·Stella Elkabes, Arnaud B Nicot
Jul 21, 2015·Molecular and Cellular Endocrinology·H M GaudetE J Filardo
Mar 16, 2013·The Laryngoscope·Daquan WuXidong Cui
Jul 21, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Eric R Prossnitz, Helen J Hathaway
Apr 14, 2012·Psychoneuroendocrinology·Lisa C LoramLinda R Watkins
Mar 15, 2015·Trends in Endocrinology and Metabolism : TEM·Matthias Barton, Eric R Prossnitz
Dec 24, 2014·Scientific Reports·Matthias R MeyerEric R Prossnitz
Mar 16, 2018·International Journal of Molecular Sciences·Annalisa TrentiAndrea Cignarella
May 4, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Chiara BolegoGiulia Chinetti-Gbaguidi
Oct 20, 2015·Scientific Reports·Alessandro VillaAdriana Maggi
Sep 26, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Isabel CabasAlfonsa García-Ayala
May 8, 2016·Clinical Science·Margaret A ZimmermanSarah H Lindsey
Jun 20, 2017·American Journal of Physiology. Heart and Circulatory Physiology·Austin C BoeseMilton H Hamblin
Aug 23, 2019·World Journal of Gastroenterology : WJG·Damian JacenikEric R Prossnitz
Mar 27, 2018·Inflammation·Lauri PolariJorma Määttä
Sep 27, 2018·Human Reproduction Update·Giovanna PepeElisabetta Vegeto
Sep 26, 2020·Frontiers in Endocrinology·Christian David Hernández-SilvaAna Laura Pereira-Suárez
Mar 3, 2018·Journal of Voice : Official Journal of the Voice Foundation·Muhammet YildizOguzhan Okcu
Nov 3, 2020·Frontiers in Endocrinology·George NotasElias Castanas
Oct 17, 2020·Pain·Ivan J M BonetJon D Levine
Aug 28, 2021·International Journal of Molecular Sciences·Sheng-Dean LuoChing-Shuen Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.