Gq DREADD activation of CaMKIIa MnPO neurons stimulates nitric oxide activity.

Journal of Neurophysiology
Alexandria B MarcianteJ Thomas Cunningham

Abstract

Designer receptors exclusively activated by designer drugs (DREADDs) modify cellular activity following administration of the exogenous ligand clozapine-N-oxide (CNO). However, some reports indicate CNO may have off-target effects. The current studies investigate the use of Gq DREADDs in CaMKIIa-expressing neurons in the median preoptic nucleus (MnPO). Male Sprague-Dawley rats (250 g) anesthetized with isoflurane were stereotaxically microinjected in the MnPO with the Gq DREADD (AAV5-CaMKIIa-HM3D-mCherry) or control virus (AAV5-CaMKIIa-mCherry). Following a 2-wk recovery, rats were used for either immunohistochemical Fos analysis or in vitro patch-clamp electrophysiology. In Gq DREADD-injected rats, CNO induced significant increases in Fos staining in the MnPO and in regions that receive direct or indirect projections from the MnPO. In electrophysiological studies, CNO depolarized and augmented firing frequency in both Gq DREADD-positive neurons (Gq DREADD) as well as unlabeled MnPO neurons in slices from Gq DREADD-injected rats (Gq DREADDx). Gq DREADDx neurons also displayed increases in spontaneous postsynaptic current (sPSC) frequency in response to CNO. Additionally, CaMKIIa-positive MnPO neurons, which also express nitric ...Continue Reading

References

Oct 1, 1986·Physiology & Behavior·J P Kroon, A L Riley
Feb 23, 1999·Life Sciences·A Christopoulos, E E El-Fakahany
Jul 18, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·R J GrindstaffJ T Cunningham
Feb 24, 2001·Cellular and Molecular Life Sciences : CMLS·T KöhlerP Plésiat
Jul 8, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Wilson A SaadJ Antunes Rodrigues
Apr 23, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Magali GrobDidier Mouginot
Sep 6, 2011·European Heart Journal·Ulrich Förstermann, William C Sessa
Mar 13, 2013·The Journal of Clinical Investigation·Shalini JainJürgen Wess
Apr 30, 2013·Free Radical Biology & Medicine·Christine Helms, Daniel B Kim-Shapiro
Jun 19, 2013·Trends in Pharmacological Sciences·Jürgen WessShalini Jain
Oct 9, 2014·Annual Review of Pharmacology and Toxicology·Daniel J Urban, Bryan L Roth
Dec 19, 2014·The International Journal of Neuropsychopharmacology·Hu Zhu, Bryan L Roth
Jul 26, 2015·The International Journal of Neuropsychopharmacology·Akash GuruMelissa R Warden
Jan 9, 2016·Nature Communications·Ken-ichiro NakajimaJürgen Wess
Jan 23, 2016·Behavioral Neuroscience·Ana Paula Crestani, Jorge Alberto Quillfeldt
Feb 26, 2016·Behavioral Neuroscience·Kyle S SmithStephen V Mahler
Mar 26, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alberto J LópezMarcelo A Wood
Apr 1, 2016·Journal of Neurophysiology·Haley L NationSean D Stocker
Aug 5, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Stephen B G AbbottClifford B Saper
Nov 26, 2016·Expert Opinion on Therapeutic Targets·Neeru M Sharma, Kaushik P Patel
Jan 27, 2017·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alexander C W SmithPeter W Kalivas
Aug 5, 2017·Science·Juan L GomezMichael Michaelides
Dec 9, 2017·Frontiers in Neural Circuits·William T KeenanSamer Hattar
Mar 1, 2018·Nature·Vineet AugustineYuki Oka
Jun 2, 2018·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Wei WangZhen Yan
Aug 30, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·George E FarmerJ Thomas Cunningham
Oct 16, 2018·Cardiovascular Research·Elisabeth KaiserPeter Lipp
Nov 18, 2018·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Stephen V MahlerGary Aston-Jones
Feb 7, 2019·American Journal of Physiology. Renal Physiology·Elena MironovaJames D Stockand
Mar 15, 2019·ACS Pharmacology & Translational Science·Karen J ThompsonAndrew B Tobin
Mar 21, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Brent ShellJ Thomas Cunningham
Mar 26, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ryan C McDonoughChristopher Price

❮ Previous
Next ❯

Methods Mentioned

BETA
transfect
light microscopy

Software Mentioned

ImageJ
Clampfit
SigmaPlot

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.