PMID: 7543919Aug 1, 1995Paper

Graded changes in the response of individual human basophils to stimulation: distributional behavior of events temporally coincident with degranulation

Journal of Leukocyte Biology
D W MacGlashan

Abstract

Human basophils, stimulated with either anti-IgE antibody or formyl-methionine-leucine-phenylalanine, were examined by two measures of the cell response that may reflect degranulation. Flow cytometric measurement of either of these two measures, changes in forward scatter intensity (an indirect measure of the basophil size) or changes in the intensity of acridine orange-loaded cells (which labels basophil granules), allowed an assessment of the distribution of single cell responses. With regard to the latter technique, structures that appeared to be basophil granules were shown to metachromatically label with low concentrations of acridine orange, which has little or no effect on histamine release. During stimulation these labeled granules were lost, leading to decreased fluorescence. Changes in either the forward scatter parameter or acridine orange labeling occurred on the same time scale as histamine release, differentiating these measures of the basophil response from early signal transduction events. Challenging basophils with a combination of phorbol myristate acetate and ionomycin caused 100% histamine release and allowed a measurement of the maximum change in forward scatter intensity or loss of acridine orange labeling...Continue Reading

Citations

Jun 17, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K VasagarS S Saini
Oct 30, 2008·Journal of Immunotoxicology·Petra A WachholzIan Kimber
Jul 14, 2006·International Archives of Allergy and Immunology·Jörg Kleine-TebbeBernhard F Gibbs
May 4, 2006·Current Opinion in Allergy and Clinical Immunology·Wayne G Shreffler
May 20, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S M ErdmannB Sachs
Apr 11, 2014·Human Vaccines & Immunotherapeutics·Salvatore Chirumbolo
Jul 13, 2016·Cytometry. Part B, Clinical Cytometry·N CopD G Ebo
Feb 1, 2005·Journal of Immunological Methods·Natalia Vilariño, Donald MacGlashan

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