Graded expression of immunomolecules on activated microglia in the hippocampus following ischemia in a rat model of ischemic tolerance

Brain Research
H KatoYasuto Itoyama

Abstract

Preconditioning of the brain with sublethal ischemia protects against neuronal damage following subsequent longer periods of ischemia (ischemic tolerance). In order to evaluate the potential involvement of microglial activation in ischemic tolerance, we immunohistochemically visualized microglial cells in the hippocampus in a rat model of ischemic tolerance. Three minutes of forebrain ischemia (preconditioning ischemia) or sham operation was followed by 6 min of ischemia (second ischemia) 3 days later. The brains were perfusion-fixed after 2 h, 1 day, 3 days, and 7 days. Microglial cells were localized by histochemical staining with isolectin-B4 from Griffonia simplicifolia and by immunohistochemistry of immunomolecules with monoclonal antibodies against major histocompatibility complex class I (OX18) and class II (OX6) antigens and complement receptor type 3 (OX42) and with a rat macrophage marker ED1 and a pan-T cell marker W3/13. Quiescent microglia were stained only by OX42. Preconditioning ischemia led to moderate microglial activation as shown by staining with isolectin, OX42, and OX18. Six minutes of ischemia (without preconditioning) caused early generalized microglial activation as shown by lectin and OX42 after 2 h an...Continue Reading

Citations

Feb 11, 2000·Brain Pathology·H Kato, W Walz
Apr 23, 2004·Expert Opinion on Therapeutic Targets·Kevin Pong
Sep 21, 2007·Journal of Neuropathology and Experimental Neurology·Sarah M McKayElspeth M McLachlan
Feb 28, 2008·Journal of Neural Transmission·M SakumaT Araki
Feb 11, 2000·Annals of the New York Academy of Sciences·J A EllisonG Z Feuerstein
Mar 11, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Masayuki Ueda, Thaddeus S Nowak
May 8, 2007·The Journal of Comparative Neurology·Emmanuel Mbaku NguKenneth J Muller
Apr 27, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J LiuF R Sharp
Feb 13, 2003·Journal of Neuropathology and Experimental Neurology·Gary H Danton, W Dalton Dietrich
Jun 23, 2005·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Toshiki HimedaTsutomu Araki
Aug 6, 1999·Current Eye Research·K S KunertC K Dorey
Apr 1, 2000·Journal of Neuropathology and Experimental Neurology·L P McCluskey, L A Lampson
Dec 4, 2008·Journal of Molecular Neuroscience : MN·Shunsuke MatsudaTsutomu Araki
Sep 23, 2021·Biological Reviews of the Cambridge Philosophical Society·Marcus Augusto-OliveiraAlexei Verkhratsky

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