Graded hypoxia modulates the invasive potential of HT1080 fibrosarcoma and MDA MB231 carcinoma cells

Clinical & Experimental Metastasis
Patrick Subarsky, Richard P Hill

Abstract

Spatial and temporal oxygen heterogeneity exists in most solid tumour microenvironments due to an inadequate vascular network supplying a dense population of tumour cells. An imbalance between oxygen supply and demand leads to hypoxia within a significant proportion of a tumour, which has been correlated to the likelihood of metastatic dissemination in both rodent tumour models and human patients. Experimentally, it has been demonstrated that near-anoxic in vitro exposure results in transiently increased metastatic potential in some tumour cell lines. The purpose of this study was to examine the effect of graded low oxygen conditions on the invasive phenotype of human tumour cells using an in vitro model of basement membrane invasion, in which we measured oxygen availability directly at the invasion surface of the transwell chamber. Our results show a relationship between culture vessel geometry and time to achieve hypoxia which may affect the interpretation of low oxygen experiments. We exposed the human tumour cell lines, HT1080 and MDA MB231, to graded normobaric oxygen (5% O(2)-0.2% O(2)) either during or prior to in vitro basement membrane invasion to simulate conditions of intravasation and extravasation. A secondary aim ...Continue Reading

References

Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S D YoungR P Hill
Jan 1, 1970·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·J D ChapmanJ O Crookall
Jun 2, 1995·The Journal of Biological Chemistry·A P LevyM A Goldberg
May 1, 1994·Analytical Biochemistry·D E Kleiner, W G Stetler-Stevenson
Sep 1, 1996·Molecular and Cellular Biology·J A ForsytheG L Semenza
Nov 1, 1996·Chemistry & Biology·L M Coussens, Z Werb
Sep 18, 1997·Journal of the National Cancer Institute·A F Chambers, L M Matrisian
Mar 21, 1998·Cancer Letters·B P Himelstein, C J Koch
Aug 31, 1999·British Journal of Cancer·R H Begent
Feb 9, 2000·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·D M BrizelM W Dewhirst
Dec 9, 2000·Journal of the Society for Gynecologic Investigation·G M SaedM P Diamond
Sep 6, 2001·Journal of the National Cancer Institute·A LalG J Riggins
Oct 13, 2001·International Journal of Radiation Oncology, Biology, Physics·G PitsonR Hill
Nov 2, 2001·Biochemical and Biophysical Research Communications·D Y YoonO Hankinson
Feb 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A FylesR P Hill
Mar 1, 2002·British Journal of Cancer·M L TurcotteJ Allalunis-Turner
Apr 20, 2002·Circulation Research·Yaara Ben-YosefAriel Miller
May 7, 2002·Nature Reviews. Cancer·Mikala Egeblad, Zena Werb
Aug 3, 2002·Nature Reviews. Cancer·Ann F ChambersIan C MacDonald
Feb 13, 2003·Advances in Experimental Medicine and Biology·L Isabel Cárdenas-NaviaMark Dewhirst
May 14, 2003·Clinical & Experimental Metastasis·Patrick Subarsky, Richard P Hill
Sep 18, 2003·Nature Reviews. Cancer·Gregg L Semenza
Jul 16, 2004·Seminars in Radiation Oncology·Michael MilosevicRichard Hill
Sep 3, 2004·Cancer Research·L Isabel Cárdenas-NaviaMark W Dewhirst
Sep 7, 2004·The International Journal of Developmental Biology·Adriana AlbiniClaudio Brigati
Nov 24, 2004·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Michael FählingBernd-Joachim Thiele
Apr 12, 2005·Cancer Science·Hiroshi SatoHisashi Miyamori
Nov 1, 2005·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Kjetil G BrurbergEinar K Rofstad
Jan 6, 2006·Radiation Research·Kjetil G BrurbergEinar K Rofstad

❮ Previous
Next ❯

Citations

Jun 11, 2008·Clinical & Experimental Metastasis·Sarah Jane LuntRichard P Hill
Mar 6, 2009·Clinical & Experimental Metastasis·Su-Bo WangXian-Jun Qu
Feb 11, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Satoru NodaKosei Hirakawa
Dec 4, 2012·Journal of Internal Medicine·L Moserle, O Casanovas
Dec 25, 2012·Experimental Cell Research·Susanna TeppoPia Nyberg
May 12, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhi-Yu SongXian-Jun Qu
Jul 17, 2010·Experimental Cell Research·Yanique RattiganDebabrata Banerjee
Jul 6, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Xiying TanDecai Tang
Dec 21, 2013·Cancer Discovery·Lidia MoserleOriol Casanovas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.