Graded recruitment and inactivation of single InsP3 receptor Ca2+-release channels: implications for quantal [corrected] Ca2+release.

The Journal of Physiology
Lucian IonescuJ K Foskett

Abstract

Modulation of cytoplasmic free Ca2+ concentration ([Ca2+]i) by receptor-mediated generation of inositol 1,4,5-trisphosphate (InsP3) and activation of its receptor (InsP3R), a Ca2+-release channel in the endoplasmic reticulum, is a ubiquitous signalling mechanism. A fundamental aspect of InsP3-mediated signalling is the graded release of Ca2+ in response to incremental levels of stimuli. Ca2+ release has a transient fast phase, whose rate is proportional to [InsP3], followed by a much slower one even in constant [InsP3]. Many schemes have been proposed to account for quantal Ca2+ release, including the presence of heterogeneous channels and Ca2+ stores with various mechanisms of release termination. Here, we demonstrate that mechanisms intrinsic to the single InsP3R channel can account for quantal Ca2+ release. Patch-clamp electrophysiology of isolated insect Sf9 cell nuclei revealed a consistent and high probability of detecting functional endogenous InsP3R channels, enabling InsP3-induced channel inactivation to be identified as an inevitable consequence of activation, and allowing the average number of activated channels in the membrane patch (N(A)) to be accurately quantified. InsP3-activated channels invariably inactivated,...Continue Reading

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Citations

Apr 13, 2010·Pflügers Archiv : European journal of physiology·J Kevin Foskett
Oct 14, 2011·Nature·Horia VaisDon-On Daniel Mak
Nov 14, 2007·The Journal of General Physiology·Lucian IonescuDon-On Daniel Mak
Nov 17, 2010·The Journal of General Physiology·Horia VaisDon-On Daniel Mak
Nov 14, 2012·The Journal of General Physiology·Horia VaisDon-On Daniel Mak
Oct 29, 2010·Cold Spring Harbor Perspectives in Biology·Colin W Taylor, Stephen C Tovey
Apr 13, 2007·Physiological Reviews·J Kevin FoskettDon-On Daniel Mak
Jan 15, 2016·Free Radical Research·Madhuri ChaurasiaKulbhushan Sharma
Mar 23, 2012·Biochemical Society Transactions·Taufiq Rahman
May 27, 2014·Frontiers in Molecular Neuroscience·Kevin B Clark
Aug 24, 2016·Proceedings of the National Academy of Sciences of the United States of America·Brendan A Bicknell, Geoffrey J Goodhill
Feb 4, 2016·Scientific Reports·Claire Shuk-Kwan LeeKing-Ho Cheung
Oct 15, 2007·The Journal of Physiology
Sep 5, 2013·Cold Spring Harbor Protocols·Don-On Daniel MakJ Kevin Foskett
Sep 5, 2013·Cold Spring Harbor Protocols·Don-On Daniel MakJ Kevin Foskett
Nov 27, 2008·The European Journal of Neuroscience·Ryland W Pace, Christopher A Del Negro
Sep 17, 2009·Immunological Reviews·Colin W TaylorSaroj Velamakanni
May 26, 2006·The Journal of Biological Chemistry·Carl WhiteJ Kevin Foskett
Oct 28, 2017·Scientific Reports·George ShapovalovNatalia Prevarskaya
Sep 23, 2006·The Journal of Biological Chemistry·Damian B van RossumSolomon H Snyder
May 17, 2011·Cell Calcium·Jean-Paul DecuypereJan B Parys
Oct 29, 2011·Biochimica Et Biophysica Acta·Ana M RossiColin W Taylor

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