Gradient Index Microlens Implanted in Prefrontal Cortex of Mouse Does Not Affect Behavioral Test Performance over Time

PloS One
Seon A LeeTeresa A Murray

Abstract

Implanted gradient index lenses have extended the reach of standard multiphoton microscopy from the upper layers of the mouse cortex to the lower cortical layers and even subcortical regions. These lenses have the clarity to visualize dynamic activities, such as calcium transients, with subcellular and millisecond resolution and the stability to facilitate repeated imaging over weeks and months. In addition, behavioral tests can be used to correlate performance with observed changes in network function and structure that occur over time. Yet, this raises the questions, does an implanted microlens have an effect on behavioral tests, and if so, what is the extent of the effect? To answer these questions, we compared the performance of three groups of mice in three common behavioral tests. A gradient index lens was implanted in the prefrontal cortex of experimental mice. We compared their performance with mice that had either a cranial window or a sham surgery. Three presurgical and five postsurgical sets of behavioral tests were performed over seven weeks. Behavioral tests included rotarod, foot fault, and Morris water maze. No significant differences were found between the three groups, suggesting that microlens implantation did...Continue Reading

References

May 16, 1978·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J Szentágothai
Jul 1, 1997·Developmental Biology·L ZhuoA Messing
Dec 15, 2000·Archives of Neurology·H X Deng, T Siddique
Dec 4, 2001·Journal of Neurophysiology·C Gonzalez-Islas, J J Hablitz
Jun 21, 2003·Optics Letters·Juergen C Jung, Mark J Schnitzer
Dec 12, 2003·Journal of Neurophysiology·Michael J LeveneWatt W Webb
Apr 29, 2004·Nature Neuroscience·György Buzsáki
May 7, 2004·Journal of Neurophysiology·Juergen C JungMark J Schnitzer
Apr 14, 2005·Bioinformatics·Eain C J GreenJohn M Hancock
Jul 30, 2005·Experimental Neurology·Michelle L StarkeyElizabeth J Bradbury
Sep 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jason N D KerrFritjof Helmchen
Apr 5, 2007·Nature Protocols·Charles V Vorhees, Michael T Williams
Apr 28, 2009·Nature·Vikaas S SohalKarl Deisseroth
Jun 16, 2009·Nature Methods·Robert P J BarrettoMark J Schnitzer
Apr 16, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Edward GlasscockJeffrey L Noebels
Dec 31, 2010·Nature Methods·Karl Deisseroth
Jan 18, 2011·Nature Medicine·Robert P J BarrettoMark J Schnitzer
Apr 8, 2011·Frontiers in Neuroscience·Rachel JamesFrancis G Szele
Jun 10, 2011·Journal of Neurophysiology·B T Thomas YeoRandy L Buckner
Jun 23, 2011·Journal of Cell Science·Isabelle ComteFrancis G Szele
Feb 22, 2012·Contrast Media & Molecular Imaging·Philippe MassotEric Thiaudiere
Apr 3, 2012·Journal of Biomedical Optics·Teresa A Murray, Michael J Levene
Apr 12, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hajime TakanoDouglas A Coulter
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Nov 13, 2012·Cold Spring Harbor Perspectives in Medicine·Marina R Picciotto, Paul J Kenny
Jul 6, 2013·Regenerative Medicine·Eric M GoldBrian J Cummings
Dec 7, 2013·Revista brasileira de psiquiatria : orgão oficial da Associação Brasileira de Psiquiatria, Asociación Psiquiátrica de la América Latina·Samira S ValvassoriJoão Quevedo
Jan 15, 2014·PloS One·Rachel K RoweJonathan Lifshitz
Mar 14, 2014·PloS One·Thomas BirngruberFrank Sinner
Dec 17, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ryan J LowDavid W Tank

❮ Previous
Next ❯

Citations

May 21, 2016·Frontiers in Neuroscience·Vladislav VoziyanovTeresa A Murray
Aug 22, 2018·Journal of Neural Engineering·L Nathan PerkinsTimothy J Gardner
Apr 27, 2019·Frontiers in Cellular Neuroscience·Daniel Aharoni, Tycho M Hoogland
Aug 28, 2020·Frontiers in Neuroscience·Sarah MalvautArmen Saghatelyan
Dec 2, 2020·Journal of Neuroscience Methods·Brenton T LaingYeka Aponte

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic

Software Mentioned

Volocity
Image J
DP Manager

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Upsala Journal of Medical Sciences
Gudrun Andrea FridgeirsdottirFredrik Clausen
Contemporary Topics in Laboratory Animal Science
Guy B Mulder, Kathleen Pritchett
© 2022 Meta ULC. All rights reserved