Gradients of a ubiquitin E3 ligase inhibitor and a caspase inhibitor determine differentiation or death in spermatids

Developmental Cell
Yosef KaplanEli Arama

Abstract

Caspases are executioners of apoptosis but also participate in a variety of vital cellular processes. Here, we identified Soti, an inhibitor of the Cullin-3-based E3 ubiquitin ligase complex required for caspase activation during Drosophila spermatid terminal differentiation (individualization). We further provide evidence that the giant inhibitor of apoptosis-like protein dBruce is a target for the Cullin-3-based complex, and that Soti competes with dBruce for binding to Klhl10, the E3 substrate recruitment subunit. We then demonstrate that Soti is expressed in a subcellular gradient within spermatids and in turn promotes proper formation of a similar dBruce gradient. Consequently, caspase activation occurs in an inverse graded fashion, such that the regions of the developing spermatid that are the last to individualize experience the lowest levels of activated caspases. These findings elucidate how the spatial regulation of caspase activation can permit caspase-dependent differentiation while preventing full-blown apoptosis.

References

Jan 1, 1972·Zeitschrift für Zellforschung und mikroskopische Anatomie·K T TokuyasuR W Hardy
May 11, 1995·Nature·S PitnickT A Markow
Feb 7, 1997·Cell·M D JacobsonM C Raff
Nov 21, 1998·Nature·M Raff
Dec 10, 1999·Molecular Biology of the Cell·J L HicksM Bownes
Feb 24, 2001·Genome Biology·J N Tittel, H Steller
Feb 19, 2002·Genes & Development·Matti DavisYinon Ben-Neriah
Mar 28, 2002·Physiological Reviews·Michael H Glickman, Aaron Ciechanover
Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Eric H Baehrecke
Dec 4, 2003·Current Opinion in Cell Biology·Andreas BergmannMayank Srivastava
Apr 9, 2004·The EMBO Journal·Lionel PintardMatthias Peter
May 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Wei YanMartin M Matzuk
Jun 18, 2004·Molecular Cell·Till BartkeStefan Jentsch
Aug 10, 2004·Nature Cell Biology·Yanyan HaoMikihiko Naito
Sep 2, 2004·Nature Reviews. Molecular Cell Biology·Timothy Cardozo, Michele Pagano
Dec 2, 2004·Biochimica Et Biophysica Acta·Andrew R WillemsMike Tyers
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Matthew D Petroski, Raymond J Deshaies
Mar 31, 2005·Seminars in Cell & Developmental Biology·Viravuth P Yin, Carl S Thummel
Jun 10, 2006·Trends in Cell Biology·June-Tai WuCheng-Ting Chien
Aug 24, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Gad AsherYosef Shaul
Oct 13, 2006·The Biochemical Journal·James H HurleyGali Prag
Mar 21, 2007·Genes & Development·Janet L Burton, Mark J Solomon
Feb 20, 2008·Cell Division·Dimple R Bosu, Edward T Kipreos
Apr 25, 2008·Development·Carine BarreauHelen White-Cooper
Apr 29, 2008·Advances in Experimental Medicine and Biology·Guy S Salvesen, Stefan J Riedl
Jan 22, 2009·Developmental Cell·Caroline H Yi, Junying Yuan
Jan 22, 2009·Developmental Cell·Liviu A MalureanuJan M van Deursen
Feb 6, 2009·Cellular and Molecular Life Sciences : CMLS·J MerletL Pintard
Feb 17, 2009·Molecular Cell·Zhijian J Chen, Lijun J Sun
Apr 18, 2009·Apoptosis : an International Journal on Programmed Cell Death·Yael Feinstein-Rotkopf, Eli Arama

❮ Previous
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Citations

Jul 4, 2012·Molecular Neurobiology·Susana SoláCecília M P Rodrigues
Sep 3, 2011·Cell Death and Differentiation·C Domingues, H D Ryoo
Mar 31, 2011·Nature Reviews. Molecular Cell Biology·Caroline GrabbeIvan Dikic
Aug 6, 2013·Genes & Development·Alejandro D'BrotJohn M Abrams
Feb 23, 2011·Development, Growth & Differentiation·Erina Kuranaga
Apr 9, 2015·Molekuliarnaia biologiia·G L Kogan, V A Gvozdev
May 28, 2014·The Journal of Cell Biology·Lindsay DeVorkinSharon M Gorski
Feb 22, 2012·The Journal of Cell Biology·Anat Florentin, Eli Arama
Jan 12, 2013·EMBO Reports·Vivek A RudrapatnaRoss L Cagan
Jan 17, 2012·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Erina Kuranaga
Feb 22, 2012·Experimental Cell Research·Flavie StrappazzonFrancesco Cecconi
Mar 19, 2015·Journal of Insect Physiology·A AccorsiD Malagoli
Oct 23, 2012·Spermatogenesis·Cindy LimXin Chen
Oct 23, 2012·Spermatogenesis·Lacramioara Fabian, Julie A Brill
Jul 17, 2012·Trends in Neurosciences·Marcello D'AmelioFrancesco Cecconi
Jul 3, 2013·Biochimica Et Biophysica Acta·Donna DentonSharad Kumar
Nov 15, 2011·Cell·Yaron Fuchs, Hermann Steller
Mar 19, 2014·Seminars in Cell & Developmental Biology·John H RichburgShawn B Bratton
May 6, 2014·Frontiers in Physiology·Patrick F ConnollyHoward O Fearnhead
May 27, 2016·Nature·Shigekazu Nagata
Aug 16, 2016·Cell Death and Differentiation·Lisa KoerverMeike Broemer
May 20, 2017·Cell Death and Differentiation·Yu-Ichiro Nakajima, Erina Kuranaga
Jan 1, 2013·Cells·Jean Berthelet, Laurence Dubrez
May 23, 2014·Nature Communications·Nicolas TerraponJürgen Liebig
Jan 3, 2019·Molecular Biology of the Cell·Céline AugièreBénédicte Durand
Mar 7, 2019·Royal Society Open Science·Ho Man Tang, Ho Lam Tang
Nov 6, 2018·International Journal of Molecular Sciences·Mohamed A EldeebMohamed A Ragheb
Sep 27, 2018·BMC Genomics·Viktor VedelekRita Sinka
Jul 12, 2017·Cell Death and Differentiation·Lior AramEli Arama
Dec 9, 2020·Proceedings of the National Academy of Sciences of the United States of America·Mélanie Ribeiro LopesFederica Calevro
Aug 24, 2019·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Xiaoxian ShiMeidan Ying
Feb 27, 2021·Journal of Cell Science·Victor PalaciosMichael Buszczak
Jul 20, 2021·Developmental Dynamics : an Official Publication of the American Association of Anatomists·David D Lowe, Denise J Montell
Aug 27, 2021·The Journal of Reproduction and Development·Zicong HuangZhenguo Chen

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