Abstract
To investigate whether graft-versus-host reactivity (GVHR) and the graft-versus-leukemia (GVL) effect can be differentiated, C3H-->AKR mixed bone marrow (BM) chimeras were prepared using two different conditioning regimens. Total body irradiation (TBI) chimeras were induced by infusing 5 x 1O(6) T cell depleted syngeneic AKR BM cells together with 15 x 1O(6) non T cell depleted allogeneic C3H BM cells 1 day after a single fraction of 10.5 Gy TBI. Total lymphoid irradiation (TLI) chimeras were prepared by injecting only 15 x 10(6) non T cell depleted C3H BM cells after 10 daily fractions of 2 Gy TLI Both groups of chimeras were healthy, without clinical or histological signs of graft-versus-host disease (GVHD). In both groups, clonal deletion of antihost-reactive donor type T lymphocytes was found. Whereas TBI chimeras did resist rejection by host type splenocytes injected 2 months after transplantation, TLI chimeras did not. As the latter phenomenon is believed to reflect remaining GVHR, TLI chimeras did have a lower remaining GVHD capacity than TBI chimeras. Nevertheless, TLI chimeras survived significantly longer after host type leukemia challenge (injection of 6 x 10(6) AKR lymphoma cells). The better survival of the TLI chi...Continue Reading
References
Apr 24, 1975·The New England Journal of Medicine·E D ThomasC D Buckner
Oct 11, 1979·Nature·M M BortinF H Bach
Mar 7, 1991·The New England Journal of Medicine·J L Ferrara, H J Deeg
Jul 3, 1990·Journal of Immunological Methods·P L LeenaretsM Waer
Jan 1, 1990·Bone·A Vesterby
Nov 1, 1985·Transplantation·A V LeFeverC C Shih
Aug 1, 1988·The Journal of Experimental Medicine·M SykesD H Sachs
Oct 1, 1987·Bone Marrow Transplantation·J PeralvoA M Marmout
Mar 1, 1986·Transplantation·R L TruittA V LeFever
Jan 1, 1986·Annual Review of Immunology·S A Rosenberg, M T Lotze
Sep 1, 1986·Transplantation·M M Bortin, A A Rimm
Apr 1, 1983·Transplantation·R F Meredith, J P Okunewick
Aug 1, 1980·The American Journal of Medicine·H M ShulmanE D Thomas
Jun 1, 1982·Cellular Immunology·L WeissS Slavin
Aug 15, 1994·Transplantation·R KorngoldT Manser
Mar 24, 1994·The New England Journal of Medicine·J O Armitage
Jan 1, 1994·Transplant International : Official Journal of the European Society for Organ Transplantation·A SalamM Waer
Citations
Mar 27, 2001·Transplantation Proceedings·A D BilliauM Waer
Aug 15, 2002·Transplantation Proceedings·P XekoukiM N Sechas
Feb 14, 1998·Nature Medicine·Y LinM Waer
May 18, 2007·Leukemia·S FeveryP Vandenberghe
Jul 6, 2001·Scandinavian Journal of Immunology·E EnghB Rolstad
Jun 27, 1997·Transplantation·Y LinM Waer
Jan 9, 1998·Transplantation·Y LinM Waer
Aug 10, 2000·Transplantation·H SefriouiM Waer
Feb 24, 2001·Transplantation·A D BilliauM Waer
May 26, 1999·Leukemia & Lymphoma·E J KresslerE R Panzer-Grümayer
Oct 31, 2002·Blood·Shawn M RigbyElizabeth H Field