Grafting of poly(ethylene glycol) onto poly(acrylic acid)-coated glass for a protein-resistant surface

Analytical Chemistry
Tetsuichi WazawaShigeru Aoyama

Abstract

The surface of solid glass supports for samples in optical microscopy and for biosensors needs to be protein-resistant. A coating of a poly(ethylene glycol) monomethyl ether (mPEG) on the surface of the glass is one promising method for preventing the nonspecific adsorption of proteins. In this study, we have developed a novel technique for achieving an optimal coverage of a glass surface with mPEG to prevent protein adhesion. A clean glass substrate previously treated with (3-aminopropyl)dimethylethoxysilane (APDMES) was treated sequentially with poly(acrylic acid) and subsequently a primary amine derivative of mPEG in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. The resultant glass surface was demonstrated to be highly protein-resistant, and the adsorption of bovine serum albumin decreased to only a few percentage points of that on a glass surface treated with APDMES alone. Furthermore, to extend the present method, we also prepared a glass substrate on which biotinylated poly(ethylene glycol) was cografted with mPEG, and biotinylated myosin subfragment-1 (biotin-S1) was subsequently immobilized on this substrate by biotin/avidin chemistry. Actin filaments were observed to glide on the biotin-S1-coated glass...Continue Reading

References

Jan 3, 1997·Biochemical and Biophysical Research Communications·A H IwaneT Yanagida
Feb 29, 2000·Biophysical Journal·T WazawaT Yanagida
Jul 6, 2000·Journal of Biomedical Materials Research·N A AlcantarJ N Israelachvili
Dec 1, 2000·Journal of Colloid and Interface Science·L D White, C P Tripp
Jun 23, 2001·Biosensors & Bioelectronics·J PiehlerG Gauglitz
Mar 17, 2004·Biosensors & Bioelectronics·Kim E Sapsford, Frances S Ligler
Jan 26, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Larry D UnsworthJohn L Brash
Jun 28, 2005·Colloids and Surfaces. B, Biointerfaces·Anne Gry HemmersamFlemming Besenbacher

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Citations

Jul 30, 2011·ACS Applied Materials & Interfaces·Rebecca A ShircliffHoward M Branz
Apr 30, 2009·Journal of Biological Engineering·Jin-Hee Han, Jeong-Yeol Yoon
Jul 1, 2008·Advanced Functional Materials·Hironobu TakahashiDavid W Grainger
May 17, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Vivienne N LukAaron R Wheeler
Sep 26, 2008·Chemistry of Materials : a Publication of the American Chemical Society·Gregory M HarbersMichael J Lochhead
Jul 5, 2007·Biomacromolecules·Dimitriya BozukovaChristine Jérôme

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