PMID: 7540641Jun 9, 1995Paper

Grafting peptides onto polystyrene microplates for ELISA

Journal of Immunological Methods
A NiveleauF Troalen

Abstract

Three peptides corresponding respectively to two Epstein-Barr viral epitopes and to the c-erbB-2 oncogene product were synthesized with the aim of developing an immunoenzymatic assay. Preliminary experiments indicated that the efficiency of the assay was profoundly affected by the nature of the solid phase for each peptide. In order to optimize the assay the three peptides were covalently coupled to functionalized polystyrene microplates which were used to immobilize both haptens and nucleic acids in a previous study. The results obtained indicate that the use of the carboxylated surfaces permits the linking strategy to be adapted to each peptide. Moreover, high sensitivities (5 x 10(-10)-1 x 10(-13) M) were obtained using amounts of the peptides much lower than those used in the standard system.

References

Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T P Hopp, K R Woods
Feb 26, 1993·Journal of Immunological Methods·A NiveleauR Dante

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Citations

Aug 16, 2011·Analytical and Bioanalytical Chemistry·Yaodong ZhangTingting Hei
Nov 26, 2013·Journal of Immunological Methods·Tinashe B RuwonaMansun Law
Dec 5, 2014·Journal of Agricultural and Food Chemistry·Yun-Hwa P Hsieh, Jack A Ofori
Feb 16, 2018·BioMed Research International·Ning LiJian Huang

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