Gram-stain plus MALDI-TOF MS (Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry) for a rapid diagnosis of urinary tract infection

PloS One
Almudena BurilloE Bouza

Abstract

Microbiological confirmation of a urinary tract infection (UTI) takes 24-48 h. In the meantime, patients are usually given empirical antibiotics, sometimes inappropriately. We assessed the feasibility of sequentially performing a Gram stain and MALDI-TOF MS mass spectrometry (MS) on urine samples to anticipate clinically useful information. In May-June 2012, we randomly selected 1000 urine samples from patients with suspected UTI. All were Gram stained and those yielding bacteria of a single morphotype were processed for MALDI-TOF MS. Our sequential algorithm was correlated with the standard semiquantitative urine culture result as follows: Match, the information provided was anticipative of culture result; Minor error, the information provided was partially anticipative of culture result; Major error, the information provided was incorrect, potentially leading to inappropriate changes in antimicrobial therapy. A positive culture was obtained in 242/1000 samples. The Gram stain revealed a single morphotype in 207 samples, which were subjected to MALDI-TOF MS. The diagnostic performance of the Gram stain was: sensitivity (Se) 81.3%, specificity (Sp) 93.2%, positive predictive value (PPV) 81.3%, negative predictive value (NPV) 93...Continue Reading

References

Jan 1, 1975·American Journal of Clinical Pathology·J H Jorgensen, P M Jones
Mar 1, 1975·Lancet·D G RobinsM S Osman
Nov 1, 1985·Diagnostic Microbiology and Infectious Disease·J L Staneck
Jun 1, 1984·Diagnostic Microbiology and Infectious Disease·F V Crout, R E Tilton
Feb 1, 1980·Journal of Clinical Microbiology·R E Tilton, R C Tilton
May 7, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·R Brönnestam
Nov 1, 2000·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P YagupskyN Peled
Oct 31, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E BouzaCo-operative Group of the European Study Group on Nosocomial Infections
Apr 24, 2003·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E J KuijperJ Dankert
Jul 10, 2003·Infectious Disease Clinics of North America·Betsy Foxman, Patricia Brown
Apr 20, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Michael L Wilson, Loretta Gaido
Jun 4, 2004·BMC Urology·Walter L J M DevilléLex M Bouter
Oct 9, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·James R Johnson
Aug 2, 2006·American Journal of Clinical Pathology·Andrew St JohnChristopher P Price
May 21, 2009·The Journal of Antimicrobial Chemotherapy·Martin Sundqvist, Gunnar Kahlmeter
Mar 26, 2010·The Lancet Infectious Diseases·Gabrielle J WilliamsJonathan C Craig
Aug 20, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L FerreiraJ M González-Buitrago
Dec 9, 2010·Nature Reviews. Urology·Betsy Foxman
Dec 31, 2010·Medical Decision Making : an International Journal of the Society for Medical Decision Making·Warren J McIsaac, Cheryl L Hunchak
May 11, 2011·Mass Spectrometry Reviews·Yen-Peng Ho, P Muralidhar Reddy
Nov 15, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A J M LoonenA J C van den Brule
Mar 1, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D MartinyO Vandenberg
Dec 12, 2012·Enfermedades infecciosas y microbiología clínica·Yanick Hoyos-MallecotJosé María Navarro-Marí
Dec 25, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Olivier ClercGilbert Greub
Aug 1, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Angela M HuangJerod L Nagel
Oct 12, 2013·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Sarah HartleyScott A Flanders
Nov 19, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·B Rodríguez-SánchezE Bouza

Citations

Nov 29, 2014·BMC Infectious Diseases·Almudena Burillo, E Bouza
Mar 5, 2016·Future Microbiology·Etienne RuppéJacques Schrenzel
Mar 5, 2015·Proteomics. Clinical Applications·Judy GopalHui-Fen Wu
Aug 25, 2015·Frontiers in Microbiology·Neelja SinghalJugsharan S Virdi
Jun 10, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L VeronF Mallard
May 11, 2015·Enfermedades infecciosas y microbiología clínica·Juan Luis Muñoz Bellido, José Manuel González Buitrago
Mar 12, 2015·BioTechniques·Laurie Vingataramin, Eric H Frost
Mar 2, 2017·Nature Reviews. Urology·Michael DavenportJoseph C Liao
Jan 22, 2017·Enfermedades infecciosas y microbiología clínica·María Siller-RuizJ L Muñoz-Bellido
Jun 27, 2017·Journal of Clinical Laboratory Analysis·Ji Seon ChoiSeong-Hyeok Choi
May 11, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Abed AthamnaSarit Freimann
Sep 15, 2018·Annals of Laboratory Medicine·Matthijs Oyaert, Joris Delanghe
Mar 27, 2020·Journal of Basic Microbiology·Chellaiah Shiny MatildaChittibabu Shanthi
Jan 26, 2017·The Journal of Antimicrobial Chemotherapy·Marina OviañoGermán Bou
Jan 9, 2019·Frontiers in Microbiology·Snehal R JadhavEnzo A Palombo
Sep 15, 2016·Expert Review of Anti-infective Therapy·Moritz FritzenwankerFlorian M Wagenlehner

Related Concepts

Gram's stain
Gentiaanviolet FNA
Phenazines
Urinary Tract Infection
Urine
Likelihood Functions
Surface Enhanced Laser Desorption Ionization Mass Spectrometry
Allergens
Antibiotics
Gram-Negative Bacteria

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Absence Epilepsy

Absence epilepsy is a common seizure disorder in children which can produce chronic psychosocial sequelae. Discover the latest research on absence epilepsies here.

Related Papers

Revista latino-americana de enfermagem
Danielle Cristina Alves FeitosaCristina Maria Garcia de Lima Parada
Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
Louis D SaravolatzBradley Belian
© 2021 Meta ULC. All rights reserved