PMID: 7541464Apr 1, 1995Paper

Gramicidin-perforated patch recording: GABA response in mammalian neurones with intact intracellular chloride

The Journal of Physiology
S EbiharaN Akaike

Abstract

1. By the development of a new perforated patch method using gramicidin, the effects of GABA on neurones dissociated from the rat substantia nigra pars reticulata (SNR) were examined without disturbing the intracellular chloride concentration. 2. Using the patch pipette solution containing gramicidin (100 micrograms ml-1), the access resistance dropped to less than 20 M omega within 40 min after making the gigaohm seal. 3. Under current-clamp conditions, GABA caused a hyperpolarization accompanied by a blockade of spontaneous firing. Under voltage clamp at a holding potential (Vh) of -50 mV, GABA evoked an outward current by way of bicuculline- and picrotoxin-sensitive GABAA receptors. 4. A 10-fold change of extracellular chloride concentration resulted in a 58 mV shift of the reversal potential of GABA-induced outward current (EGABA), indicating that the membrane behaves like a chloride electrode in the presence of GABA. 5. The intracellular chloride activities (aCli), calculated with the Nernst equation using both extracellular chloride activity and EGABA values, ranged from 2.8 to 19.7 mM with a mean value of 9.5 mM. The aCli was not affected either by different pipette solutions or by different holding potentials more hyper...Continue Reading

Citations

Jul 19, 2000·Journal of Neurophysiology·Y KakazuJ Nabekura
Jan 15, 2002·American Journal of Physiology. Cell Physiology·Masahiko ShimuraNobutoshi Harata
Nov 29, 2002·American Journal of Physiology. Cell Physiology·Sok Han KangTerence K Smith
Nov 16, 2002·The Journal of Physiology·Daniela Billups, David Attwell
Dec 21, 2002·The European Journal of Neuroscience·Carmen Vale, Dan H Sanes
Sep 1, 2005·Annals of Neurology·Jie WuJohn F Kerrigan
May 6, 2014·Frontiers in Cellular Neuroscience·Chang-Hoon Cho
Jan 7, 2016·Annals of Clinical and Translational Neurology·Joseph Glykys, Kevin J Staley
Jan 6, 1999·The European Journal of Neuroscience·J Brockhaus, K Ballanyi
Dec 4, 2001·Journal of Neurophysiology·M MartinaD Paré
Feb 24, 2004·Visual Neuroscience·Katalin RablWallace B Thoreson
Oct 12, 2007·Physiological Reviews·Yehezkel Ben-AriRustem Khazipov
Apr 4, 2012·Current Protocols in Neuroscience·Ulrike Klenke, Carol Taylor-Burds
Aug 12, 2014·The Journal of Physiology·Eric Delpire, Kevin J Staley
Oct 29, 2014·Journal of Applied Toxicology : JAT·Rahul R KauthaleMadhumanjiri M Gatne
Apr 5, 2017·The Journal of Clinical Investigation·Xiaona DuNikita Gamper
Jul 28, 2017·Journal of Neurophysiology·Cory A Massey, George B Richerson
Mar 11, 2000·American Journal of Physiology. Endocrinology and Metabolism·F Le FollL Cazin
Mar 4, 2003·Journal of Neurophysiology·Colleen R Shields, Peter D Lukasiewicz
Sep 1, 1997·The Journal of General Physiology·H F Clemo, C M Baumgarten
Jul 18, 2003·Journal of Neurophysiology·Roman TyzioRoustem Khazipov
Jun 19, 2001·The Journal of Physiology·J F van BrederodeC Alzheimer
Apr 4, 2006·Nature Neuroscience·Kenichi N HartmanVenkatesh N Murthy
Oct 6, 2007·Annals of Neurology·Volodymyr I DzhalaKevin J Staley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.