Gramicidin-perforated patch recording revealed the oscillatory nature of secretory Cl- movements in salivary acinar cells

The Journal of General Physiology
M SugitaY Shiba

Abstract

Elevations of cytoplasmic free calcium concentrations ([Ca(2+)](i)) evoked by cholinergic agonists stimulate isotonic fluid secretion in salivary acinar cells. This process is driven by the apical exit of Cl(-) through Ca(2+)-activated Cl(-) channels, while Cl(-) enters the cytoplasm against its electrochemical gradient via a loop diuretic-sensitive Na(+)-K(+)-2Cl(-) cotransporter (NKCC) and/or parallel operations of Cl(-)-HCO(3)(-) and Na(+)-H(+) exchangers, located in the basolateral membrane. To characterize the contributions of those activities to net Cl(-) secretion, we analyzed carbachol (CCh)-activated Cl(-) currents in submandibular acinar cells using the "gramicidin-perforated patch recording configuration." Since the linear polypeptide antibiotic gramicidin creates monovalent cation-selective pores, CCh-activated Cl(-) currents in the gramicidin-perforated patch recording were carried by Cl(-) efflux via Cl(-) channels, dependent upon Cl(-) entry through Cl(-) transporters expressed in the acinar cells. CCh-evoked oscillatory Cl(-) currents were associated with oscillations of membrane potential. Bumetanide, a loop diuretic, decreased the CCh-activated Cl(-) currents and hyperpolarized the membrane potential. In contr...Continue Reading

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Citations

Sep 16, 2009·Biophysical Journal·Nicolás Montalbetti, Jorge Fischbarg
Jul 1, 2005·American Journal of Physiology. Cell Physiology·Edward M Blumenthal
Jan 12, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Takahide ShintaniYoshiki Shiba
Jan 4, 2019·International Journal of Oral Science·Yuichiro KusudaRyuji Hosokawa
Jun 7, 2006·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Chikara Hirono

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