Abstract
Trials evaluating granulocyte transfusions for therapy of febrile neutropenia demonstrated mixed clinical results, which may in part be due to the low neutrophil cell dose of the transfused components. To optimize the latter variable, the effect of various interface offset (IO) settings during apheresis on granulocyte collection efficiency (GCE) and yield was determined in normal donors given granulocyte-colony-stimulating factor (G-CSF). Seventeen donors underwent 44 granulocyte collections performed with a continuous-flow blood cell separator with hetastarch infusion after stimulation with G-CSF (5 micrograms/kg/day). Apheresis was performed alternatively using one of three IO settings: IO = 15 (Group 1, n = 16), IO = 25 (Group 2, n = 12), and IO = 35 (Group 3, n = 13). The GCE and yield and changes in donor platelet count, hematocrit (Hct), and red cell (RBC) volume of the components were compared among the three groups. The mean GCE was greater (p = 0.006) during apheresis at an IO setting of 35 (60 +/- 12%) than at an IO setting of 15 (40 +/- 17%). The mean granulocyte yield (x10(10) per procedure was 10.8 +/- 2.95 at an IO setting of 35 and 7.73 +/- 4.02 at an IO setting of 15 (p = 0.08). In a multivariate linear regressi...Continue Reading
Citations
Feb 11, 2010·International Journal of Hematology·Akimichi OhsakaUNKNOWN Japan Society of Transfusion Medicine and Cell Therapy, Granulocyte Transfusion Task Force
Apr 29, 1999·Transfusion·P StohlawetzB Jilma
Sep 16, 2000·Transfusion·T LightfootD F Stroncek
Oct 12, 2002·Transfusion·H G HeuftR Blasczyk
Feb 17, 2000·Current Opinion in Pediatrics·D C Dale, W C Liles
Jan 23, 1999·Current Opinion in Hematology·C A Schiffer
Apr 12, 2005·Transfusion·Hans-Gert HeuftRainer Blasczyk
Oct 7, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Uwe SchwankeRainer Moog
Jun 10, 2005·Transfusion Medicine·U SchwankeR Moog
Jun 24, 2004·Vox Sanguinis·H EinseleB Neumeister
Oct 20, 2004·Journal of Clinical Apheresis·Rainer Moog
Nov 11, 2011·Journal of Clinical Apheresis·Kian KesslerHans-Gert Heuft
Apr 24, 2012·Transfusion·Junko IkemotoShunro Kai
Apr 16, 2013·Transfusion·Kristin ThorauschHalvard Bonig
Dec 22, 2000·Blood Reviews·D B Jendiroba, E J Freireich
May 10, 2005·Journal of Clinical Apheresis·Edwin A Burgstaler