Granulocyte colony-stimulating factor and bone marrow mononuclear cells for stroke treatment in the aged brain

Current Neurovascular Research
Ana-Maria BugaA Popa-Wagner

Abstract

Ischemic stroke swiftly induces a wide spectrum of pathophysiological sequelae, particularly in the aged brain. The translational failure of experimental therapies, might partially be related to monotherapeutic approaches, not address potential counter-mechanisms sufficiently or within the best time window. For example, therapeutic effects relying on stem/progenitor cell mobilization by granulocyte-colony stimulating factor (G-CSF), require approximately a week to become manifest, which is potentially beyond the optimal timing. Here, We tested the hypothesis that treating post-stroke aged rats with the combination of bone marrow-derived mononuclear cells (BM MNC) and G-CSF improves the long term (56 days) functional outcome by compensating the delay before G-CSF effects come to full effect. 1x10(6) syngeneic BM MNC per kg bodyweight (BW) with G-CSF (50 µg/kg, given intraperitoneal by via the jugular vein to aged Sprague- Dawley rats, six hours post-stroke. This process was repeated daily, for a 28 day period. Infarct volume was measured by magnetic resonance imaging at 3 and 48 days post-stroke and additionally by immunohistochemistry at day 56. Functional recovery was tested during the entire post-stroke survival period. Daily...Continue Reading

Citations

Dec 5, 2019·International Journal of Molecular Sciences·Roxana SurugiuAurel Popa-Wagner
Sep 28, 2018·Stem Cell Research & Therapy·Syed Shadab RazaMohsin Ali Khan
Dec 6, 2019·Frontiers in Neuroscience·Daniel HenriquesLiliana S Mendonça
Oct 15, 2020·International Journal of Molecular Sciences·Dejan NikolicIvana Novakovic

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