Granulocyte colony-stimulating factor prophylaxis improves survival and inflammation in a two-hit model of hemorrhage and sepsis

Critical Care Medicine
Artur BauhoferAlexander Torossian

Abstract

We evaluated the effects of a granulocyte-colony stimulating factor (G-CSF) prophylaxis in two clinically relevant situations, hemorrhage on the day before infection (e.g., trauma) and acute hemorrhage followed subsequently by infection (e.g., operative complication). A two-hit model of hemorrhage and polymicrobial peritoneal contamination and infection (PCI) was used to assess the influence of G-CSF on the outcome, bacterial clearance, and cytokine pattern. Clinic modeling randomized laboratory trial. University laboratory. One hundred thirty-two male rats. In trial 1 we compared a) preoperative PCI only; b) preoperative hemorrhage plus PCI; and c) hemorrhage plus PCI plus G-CSF prophylaxis (n=18 rats/group). In trial 2, intraoperative hemorrhage was assessed with the same trial design. Primary end point was survival at 120 hrs. In trial 2 additionally, six rats per group and six naive control rats were used for secondary end point analysis. Primary end point was mortality at 120 hrs. Secondary end points were granulocyte counts, bacterial clearance, and local cytokine levels. In trial 1 survival rate was 56% after PCI only, 17% after hemorrhage plus PCI, and 61% after hemorrhage plus PCI plus G-CSF (p<.01). In trial 2 surviva...Continue Reading

References

Mar 1, 1976·The American Journal of Physiology·P A Johnston, E E Selkurt
Jan 1, 1991·The American Journal of Physiology·G BoyarskyW F Boron
Aug 1, 1988·Circulation Research·J Barroso-ArandaR L Engler
Jan 1, 1986·New York State Journal of Medicine·L N Robinson
Oct 25, 1984·The New England Journal of Medicine·R L NicholsJ Mills
Jun 1, 1994·Brain, Behavior, and Immunity·M R ShurinB S Rabin
Feb 1, 1993·American Journal of Surgery·I H Chaudry, A Ayala
Mar 1, 1993·The British Journal of Surgery·R G MolloyM L Rodrick
Jun 1, 1997·Infection and Immunity·T HenslerB Holzmann
Feb 17, 2001·Journal of Applied Toxicology : JAT·K H DiehlUNKNOWN European Federation of Pharmaceutical Industries Association and European Centre for the Validation of Alternative Methods
Nov 13, 2001·Diabetes, Obesity & Metabolism·A YönemA Corakçi
Apr 16, 2002·Internal Medicine Journal·D P StephensB J Currie

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Citations

Mar 1, 2006·Critical Care Medicine·Michael Bauer, Konrad Reinhart
May 8, 2007·Journal of the American College of Surgeons·Mario PerlAlfred Ayala
Apr 10, 2007·Seminars in Fetal & Neonatal Medicine·Adam J WolfbergPierre Gressens
Dec 17, 2009·Clinical Orthopaedics and Related Research·Nikolaos G LasanianosPeter V Giannoudis
May 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Margaret L HibbsAshley R Dunn

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