Granulocyte-macrophage colony-stimulating factor activates the transcription of nuclear factor kappa B and induces the expression of nitric oxide synthase in a skin dendritic cell line

Immunology and Cell Biology
M T CruzM C Lopes

Abstract

Nitric oxide (NO) produced by skin dendritic cells and keratinocytes plays an important role in skin physiology, growth and remodelling. Nitric oxide is also involved in skin inflammatory processes and in modulating antigen presentation (either enhancing or suppressing it). In this study, we found that GM-CSF stimulates the expression of the inducible isoform of nitric oxide synthase (iNOS) in a fetal-skin-derived dendritic cell line (FSDC) and, consequently, increases the nitrite production from 11.9 +/- 3.2 micromol/L (basal level) to 26.9 +/- 4.2 micromol/L. Pyrrolidinedithiocarbamate (PDTC) inhibits nitrite production, with a half maximal inhibitory concentration (IC50) of 19.3 micromol/L and the iNOS protein expression in FSDC. In addition, western blot assays revealed that exposure of FSDC to GM-CSF induces the phosphorylation and degradation of the inhibitor of NF-kappaB (IkB), with subsequent translocation of the p50, p52 and RelB subunits of the transcription nuclear factor kappa B (NF-kappaB) from the cytosol to the nucleus. Electrophoretic mobility shift assays (EMSA) showed that FSDC exposure to GM-CSF activates the transcription factor NF-kappaB. Together, these results show that GM-CSF induces iNOS expression in s...Continue Reading

References

Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Sep 1, 1994·The Journal of Experimental Medicine·E MartinQ W Xie
Aug 1, 1995·European Journal of Immunology·G GirolomoniP Ricciardi-Castagnoli
Apr 1, 1996·European Journal of Immunology·C BlankH Moll
Dec 1, 1996·The Journal of Investigative Dermatology·A A QureshiE A Lerner
Dec 1, 1996·The Journal of Allergy and Clinical Immunology·S WatanabeK Arai
Jan 1, 1997·The British Journal of Dermatology·A RoweC B Bunker
May 29, 1997·Biochemical and Biophysical Research Communications·R LiuS Watanabe
Jul 1, 1997·American Journal of Respiratory and Critical Care Medicine·H W LiuR Kradin
Jan 7, 1998·Critical Reviews in Immunology·A ChoudhuryD F Claxton
Jan 10, 1998·The Journal of Investigative Dermatology·D Bruch-GerharzV Kolb-Bachofen
Mar 6, 1998·International Immunology·R RossA B Reske-Kunz
Apr 1, 1998·Nature·J Banchereau, R M Steinman
Jun 10, 1998·Biochemical and Biophysical Research Communications·F J ThorntonA Barbul
Dec 4, 1998·Archives of Dermatological Research·H YokozekiK Nishioka
Dec 8, 1998·The Journal of Experimental Medicine·M RescignoP Ricciardi-Castagnoli
Dec 16, 1998·Trends in Pharmacological Sciences·S LamasS Moncada
Jan 29, 2000·The Journal of Cell Biology·P Ernfors
Feb 10, 2000·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·S StephanC L Chander
Jun 1, 2000·The Journal of Cell Biology·C K Kaufman, E Fuchs

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Citations

Mar 28, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Ji Young ChaWon G An
Jun 12, 2010·Journal of Ethnopharmacology·Ana Cristina TavaresLígia Ribeiro Salgueiro
Apr 9, 2005·The Journal of Investigative Dermatology·Norikatsu MizumotoAkira Takashima
Jul 21, 2009·Immunobiology·Chiara PortaAntonio Sica
Jun 21, 2013·Journal of the American Heart Association·Chek K TanNguan S Tan
Aug 28, 2007·The Journal of Biological Chemistry·Zachary T KelleherHarvey E Marshall
Jul 28, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Heba A GheitaMona A El-Ghazaly

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