Granulocyte-macrophage colony-stimulating factor increases the infectivity of Leishmania amazonensis by protecting promastigotes from heat-induced death.

Infection and Immunity
M A BarcinskiR Charlab

Abstract

We have studied the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on the infectivity of promastigotes of Leishmania amazonensis, an obligate intramacrophage parasite. We measured the capacity of the promastigotes to infect macrophages after preincubation at different temperatures (28, 34, and 37 degrees C) with recombinant murine GM-CSF, as well as the effect of an anti-murine GM-CSF antibody on the in vitro and in vivo infectivity of the parasite. GM-CSF increases the capacity of the promastigotes to infect cells when preincubated at 34 and 37 degrees C, whereas the anti-GM-CSF antibody exerts the opposite effect: it decreases the internalization rate and the progression of infection in macrophage cultures and slows the growth of the lesion in infected BALB/c mice. Neither of the described effects were observed when the in vitro and in vivo infections were made with amastigotes. Promastigotes die in a time-dependent manner when incubated at temperatures higher than 28 degrees C in the absence of GM-CSF. They are protected from this heat-induced death by incubation with the recombinant hormone. Our interpretation of these data is that the increase in the infectivity of promastigotes when incubated with GM-...Continue Reading

References

Jun 1, 1990·Science·Y Sanchez, S L Lindquist
Feb 1, 1990·Journal of Leukocyte Biology·J Mauël
Aug 1, 1989·Molecular and Biochemical Parasitology·G HideA Tait
Aug 1, 1988·European Journal of Immunology·Z Y FengP Vassalli
Sep 1, 1986·The American Journal of Tropical Medicine and Hygiene·A Warburg, Y Schlein
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D Le RoithM A Lesniak
Mar 30, 1984·Science·D L Sacks, P V Perkins
Oct 1, 1980·The Journal of Experimental Medicine·T M DexterD Metcalf
May 1, 1990·Parasitology Today·R G Titus, J M Ribeiro
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D LeroithD T Krieger

❮ Previous
Next ❯

Citations

Apr 28, 2005·Antimicrobial Agents and Chemotherapy·Denise C ArrudaSilvia R B Uliana
Oct 5, 2006·Parasitology·S C WelburnM Duzensko
Sep 6, 2000·International Journal of Experimental Pathology·C M GomesC E Corbett
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·H GotoM Gidlund
Jun 12, 2008·PLoS Neglected Tropical Diseases·Danilo C MiguelSilvia R B Uliana
Mar 1, 1994·European Journal of Immunology·S ZimmermannW Solbach
May 29, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M A Barcinski, G A DosReis
Nov 29, 2015·Parasite Immunology·Izabel Galhardo DemarchiMaria Valdrinez Campana Lonardoni
Dec 1, 1996·Immunology and Cell Biology·M RiffkinP Wood
Dec 23, 2017·Parasite Immunology·L Sánchez-GarcíaI Becker
Dec 1, 2001·Current Biology : CB·J M de Freitas BalancoM A Barcinski
Feb 1, 2000·Parasitology Today·M Parsons, L Ruben
Jan 1, 1997·Parasitology Today·S C WelburnG T Williams
Dec 13, 2005·International Journal for Parasitology·Marta T GomesAngela H Lopes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.