Granulocyte-macrophage colony-stimulating factor (GM-CSF) primes the respiratory burst and stimulates protein biosynthesis in human neutrophils

FEBS Letters
S W EdwardsC A Hart

Abstract

Pre-treatment of human neutrophils with rGM-CSF resulted in a 3-fold increase in the rate of fMet-Leu-Phe stimulated reactive oxidant generation, as assessed by luminol- and lucigenin-chemiluminescence and O2- secretion. When blood-stream neutrophils were incubated in RPMI 1640 medium supplemented with [35S]methionine, both fMet-Leu-Phe (0.1 microM) and gamma-interferon (100 U/ml) stimulated a 3-4-fold increased incorporation of label into TCA-precipitable material. Similarly, rGM-CSF (50 U/ml) also stimulated protein biosynthesis in bloodstream neutrophils, and newly labelled polypeptides were separated by two-dimensional polyacrylamide gel electrophoresis. Two classes of polypeptides were visualised on these gels: the relative rate of labelling of one class changed very little upon rGM-CSF treatment whereas the relative rate of labelling of a second group increased 3-12-fold.

References

Apr 15, 1989·Biochemical Pharmacology·J M HumphreysS W Edwards
Feb 15, 1989·Annals of Internal Medicine·R H WeisbartD W Golde
Jun 1, 1989·Biochemical Society Transactions·S W Edwards
Dec 1, 1987·Journal of General Microbiology·S W EdwardsC A Hart
Apr 1, 1987·The Journal of Experimental Medicine·F ColottaA Mantovani
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Mar 1, 1973·The Journal of Clinical Investigation·B M BabiorJ T Curnutte

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Citations

Oct 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C RathakrishnanM L Tiku
Jul 1, 1994·Pharmaceutica acta Helvetiae·D Steinhilber
Jul 1, 1992·International Journal of Immunopharmacology·E UetaT Osaki
Feb 28, 2004·Il Farmaco·Olga BrunoFranco Dallegri
Mar 1, 1994·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·E UetaI Kato
May 1, 1993·Annals of the Rheumatic Diseases·F WatsonS W Edwards
Mar 1, 1996·Leukemia Research·Y XuC L Willman
Jan 1, 1990·International Journal of Cell Cloning·M AgliettaF Gavosto
Jan 1, 1991·The International Journal of Biochemistry·A D Whetton, S J Vallance
Sep 1, 1991·Veterinary Pathology·J M MadduxW L Sanders
Mar 1, 1994·FEMS Immunology and Medical Microbiology·J A QuayleS W Edwards

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