Granulocyte-macrophage colony-stimulating factor and B7-2 combination immunogene therapy in an allogeneic Hu-PBL-SCID/beige mouse-human glioblastoma multiforme model

Human Gene Therapy
I F ParneyL J Chang

Abstract

Glioblastoma multiforme is the most common primary central nervous system neoplasm. Its dismal prognosis has led to investigation of new treatment strategies such as immunogene therapy. We transduced the human glioblastoma cell line D54MG in vitro with genes encoding the proinflammatory cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF), the T cell co-stimulatory molecule B7-2, or both (in a bicistronic vector) via retroviral vectors. Therapeutic gene expression by D54MG was high after transduction and selection (30 ng/10(6) cells/day for GM-CSF and > 2 orders of magnitude fluorescence shift on flow cytometry for B7-2). The effect of GM-CSF and/or B7-2 transduction on D54MG tumor growth in vivo was monitored in a novel allogeneic human peripheral blood lymphocyte-severe combined immunodeficiency mouse (Hu-PBL-SCID) model. GM-CSF- or B7-2-transduced tumors showed growth suppression in hu-PBL-reconstituted mice compared to untransduced and/or unreconstituted controls. Growth suppression was greatest for B7-2. Furthermore, vaccination with irradiated GM-CSF/B7-2-transduced tumor cells markedly inhibited growth of wild-type tumors at distant sites. Thus, this study illustrates a potential gene therapy strategy for g...Continue Reading

References

Jul 1, 1992·Journal of Neurosurgery·K L BlackJ E Merrill
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M ProchazkaE H Leiter
Jul 2, 1992·The New England Journal of Medicine·G J Lieschke, A W Burgess
Dec 1, 1991·Immunological Reviews·B E TorbettD E Mosier
Mar 1, 1991·Journal of Neurosurgery·T MatsumotoK Miyaji
Aug 1, 1989·Journal of Neurosurgery·M C KuppnerN de Tribolet
Jul 26, 1968·Science·P BendaW Sweet
Feb 10, 1983·Nature·G C BosmaM J Bosma
Nov 1, 1995·Immunology Today·M Tary-LehmannP V Lehmann
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·V A BoussiotisL M Nadler
Feb 1, 1994·The Journal of Experimental Medicine·L ChenK E Hellström
Aug 1, 1994·Protein Science : a Publication of the Protein Society·P S LinsleyJ Bajorath
Nov 1, 1995·The Journal of Experimental Medicine·T C WuD M Pardoll
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S BaskarL H Glimcher
Dec 1, 1994·Current Opinion in Neurology·W K Yung
Jun 1, 1994·Journal of Leukocyte Biology·A Mondino, M K Jenkins
Mar 1, 1994·The Journal of Urology·M G SandaJ W Simons
Jun 1, 1993·Surgical Neurology·P K KornblithM K Bradley
Jul 1, 1993·Journal of Neurosurgery·Y TakamiyaR L Martuza
Nov 1, 1993·Journal of Neurosurgery·D BarbaF H Gage
Mar 1, 1993·Archives of Surgery·C Chandler, E Passaro

❮ Previous
Next ❯

Citations

Feb 5, 2003·Journal of Biomedical Science·Ian F Parney, Lung-Ji Chang
Jun 18, 2009·In Vitro Cellular & Developmental Biology. Animal·Anne ClavreulPhilippe Menei
Apr 2, 2003·Pharmacology & Therapeutics·M G CastroP R Lowenstein
Jun 29, 2001·Trends in Immunology·R B BankertS D Hess
Jun 5, 2003·Current Opinion in Oncology·Carlo MarrasFrancesco DiMeco
Aug 5, 2000·Drugs & Aging·M J PerryA G Dalgleish
Dec 15, 2010·Future Oncology·Erik Hp van PuttenMartine Lm Lamfers
Jan 1, 2010·Clinical & Developmental Immunology·Lisheng GeMartin R Jadus
Jan 11, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sizhe FengXuezhong Wei
Sep 29, 2004·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Steven De VleeschouwerFrank Van Calenbergh
Aug 28, 2002·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·Ian F ParneyKenneth C Petruk
Sep 30, 2000·Stem Cells·L CaoF M Rosenthal
Apr 21, 2006·Expert Review of Neurotherapeutics·Maciej S Lesniak
Jul 4, 2012·Neurosurgery Clinics of North America·Seunggu J HanAndrew T Parsa
Dec 1, 2009·Neurosurgery Clinics of North America·Seunggu J HanMichael Lim
Feb 20, 2007·Molecular Aspects of Medicine·Sonia MinuzzoAlberto Amadori
Apr 19, 2008·Journal of Cellular and Molecular Medicine·Baojun ZhangYong Zhao
Oct 4, 2005·Neurological Research·Christian W Sikorski, Maciej S Lesniak
Jul 10, 2014·Journal of Cancer Research and Clinical Oncology·Wei ZhouZhenyu Shao
Apr 14, 2000·Neurosurgery·I F ParneyK C Petruk
Sep 30, 2004·Gene Therapy·S S LarinS L Kiselev
Nov 13, 2013·Expert Review of Neurotherapeutics·Masoud MirghorbaniNima Rezaei
May 12, 2000·Neurosurgery·I F ParneyK C Petruk
May 6, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yueh-Chun LiChyi-Chyang Lin

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.