Granulocyte-macrophage progenitors as candidate leukemic stem cells in blast-crisis CML

The New England Journal of Medicine
Catriona H M JamiesonIrving L Weissman

Abstract

The progression of chronic myelogenous leukemia (CML) to blast crisis is supported by self-renewing leukemic stem cells. In normal mouse hematopoietic stem cells, the process of self-renewal involves the beta-catenin-signaling pathway. We investigated whether leukemic stem cells in CML also use the beta-catenin pathway for self-renewal. We used fluorescence-activated cell sorting to isolate hematopoietic stem cells, common myeloid progenitors, granulocyte-macrophage progenitors, and megakaryocyte-erythroid progenitors from marrow during several phases of CML and from normal marrow. BCR-ABL, beta-catenin, and LEF-1 transcripts were compared by means of a quantitative reverse-transcriptase-polymerase-chain-reaction assay in normal and CML hematopoietic stem cells and granulocyte-macrophage progenitors. Confocal fluorescence microscopy and a lymphoid enhancer factor/T-cell factor reporter assay were used to detect nuclear beta-catenin in these cells. In vitro replating assays were used to identify self-renewing cells as candidate leukemic stem cells, and the dependence of self-renewal on beta-catenin activation was tested by lentiviral transduction of hematopoietic progenitors with axin, an inhibitor of the beta-catenin pathway. T...Continue Reading

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C M BaumB Peault
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C UdomsakdiA C Eaves
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·D E AfarM F Roussel
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M L GishizkyO N Witte
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·N UchidaI L Weissman
Apr 29, 1999·The New England Journal of Medicine·C L Sawyers
Dec 30, 1999·Nature Genetics·C S HuettnerD G Tenen
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·T Era, O N Witte
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·T MiyamotoK Akashi
Nov 2, 2001·Nature·T ReyaI L Weissman
Feb 9, 2002·The Journal of Biological Chemistry·Mascha van NoortHans Clevers
Mar 29, 2002·Nature Immunology·Stuart H Orkin, Leonard I Zon
Apr 17, 2002·Cell·Jennifer AntonchukR Keith Humphries
Aug 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Markus G ManzIrving L Weissman
Mar 5, 2003·Annual Review of Immunology·Motonari KondoIrving L Weissman
Mar 6, 2003·Oncogene·Joyce A SchroederSandra J Gendler
Mar 15, 2003·The New England Journal of Medicine·Stephen G O'BrienUNKNOWN IRIS Investigators
Apr 30, 2003·Nature·Tannishtha ReyaIrving L Weissman
Aug 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Siddhartha JaiswalIrving L Weissman
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Emmanuelle PasseguéIrving L Weissman
Oct 10, 2003·The New England Journal of Medicine·Tim P HughesUNKNOWN International Randomised Study of Interferon versus STI571 (IRIS) Study Group
Oct 10, 2003·The New England Journal of Medicine·John M Goldman, Junia V Melo
Dec 26, 2003·The New England Journal of Medicine·Erming TianJohn D Shaughnessy

❮ Previous
Next ❯

Citations

Sep 18, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Sarah K Brennan, William Matsui
Oct 29, 2011·Cancer Immunology, Immunotherapy : CII·Michal Smahel
Jun 3, 2005·Annals of Hematology·Rüdiger HehlmannAndreas Hochhaus
Nov 26, 2010·Annals of Hematology·Ugo Testa
Aug 10, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Vincent E SollarsArthur M Buchberg
Oct 17, 2007·Cell and Tissue Research·Malcolm R AlisonSimon Leedham
May 12, 2005·Journal of Mammary Gland Biology and Neoplasia·Yi Li, Jeffrey M Rosen
May 12, 2005·Journal of Mammary Gland Biology and Neoplasia·Gabriela Dontu, Max S Wicha
Apr 27, 2012·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jian MaZuoming Sun
Nov 26, 2010·Frontiers of Medicine in China·Ying-Li WuGuo-Qiang Chen
Apr 18, 2006·Current Treatment Options in Oncology·Merat Karbasian EsfahaniPeter H Wiernik
Apr 11, 2013·Current Treatment Options in Oncology·Preetesh JainJorge Cortes
Apr 29, 2010·Current Hematologic Malignancy Reports·Mhairi Copland
Mar 31, 2011·Current Hematologic Malignancy Reports·Deborah L White, Timothy P Hughes
Apr 3, 2012·Current Hematologic Malignancy Reports·Leslie A Crews, Catriona H M Jamieson
Jan 12, 2013·Current Hematologic Malignancy Reports·Wesam Ahmed, Richard A Van Etten
Sep 29, 2006·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·José A Martínez-ClimentFelipe Prosper
Sep 6, 2006·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Anthony M Ford, Angel Martínez-Ramírez
May 31, 2008·International Journal of Hematology·Daigo AkahaneKazuma Ohyashiki
Jun 10, 2010·International Journal of Hematology·Kristen N Rice, Catriona H M Jamieson
Jul 30, 2011·International Journal of Hematology·Florian H HeidelScott A Armstrong
Apr 26, 2013·Drugs & Aging·Massimo Breccia, Giuliana Alimena
Apr 20, 2005·Cancer Letters·Julien MazieresDavid M Jablons
Mar 29, 2008·Cell Stem Cell·Ravindra MajetiIrving L Weissman
Mar 29, 2008·Cell Stem Cell·Aniruddha J Deshpande, Christian Buske
Feb 24, 2009·Bone Marrow Transplantation·T LinW Matsui
Feb 9, 2008·Cell Death and Differentiation·L VermeulenJ P Medema
Apr 9, 2008·Cell Research·Roel Nusse
Sep 19, 2008·Leukemia·N MisaghianJ A McCubrey
Mar 20, 2009·Leukemia·R NaughtonT G Cotter
Sep 24, 2010·Leukemia·I SlomaC J Eaves
Sep 17, 2010·Leukemia·D M RossJ V Melo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

CZI Human Cell Atlas Seed Network

The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.