Granulocyte phagocytosis of Streptococcus pneumoniae in properdin-deficient serum.

Infection and Immunity
J H BraconierA G Sjöholm

Abstract

Properdin (P)-deficient human serum containing type-specific anticapsular antibodies and having an intact classical pathway of complement did not support efficient granulocyte phagocytosis of Streptococcus pneumoniae serotypes 6A, 14, 19F, 23F, or 35 in an opsonophagocytic assay. Compared with pooled control serum, the difference was most pronounced for serotypes 14 and 23F and at a final serum concentration of 16%. The reduced phagocytic killing of S. pneumoniae serotype 23F in P-deficient serum was due rather to defective opsonization than to impaired intracellular killing. The uptake of C3 by the serotype 23F strain was found to be low in P-deficient serum. The addition of native P promoted C3 fixation as well as opsonization. The activity of P was abolished by heat treatment (56 degrees C, 30 min). Experiments with Mg EGTA to block C1 activation suggested that serotype 23F pneumococci were more dependent on the classical pathway for opsonization than were serotype 35 pneumococci, which appear to be partly opsonized through the alternative pathway alone.

References

Aug 1, 1977·Infection and Immunity·C G StephensW P Reed
Feb 1, 1978·Immunochemistry·J O Minta, W Vetvutanapibul
Jan 1, 1978·Medicine·V Agnello
Feb 1, 1977·The Journal of Experimental Medicine·A G Ehlenberger, V Nussenzweig
May 1, 1973·The Journal of Pediatrics·J A Winkelstein
Jan 1, 1966·International Archives of Allergy and Applied Immunology·P J McConahey, F J Dixon
Jan 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·P O Ganrot
Sep 29, 1966·The New England Journal of Medicine·F S Rosen, C A Janeway
Mar 1, 1982·Journal of Clinical Microbiology·F K Pedersen, J Henrichsen

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Citations

Oct 1, 1990·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A G Sjöholm
Jan 1, 1995·Scandinavian Journal of Infectious Diseases·K EkdahlJ H Braconier
Mar 1, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sunita GulatiSanjay Ram
Nov 1, 1991·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·C SöderströmB Thuresson
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Youssif Mohammed AliWilhelm J Schwaeble
Sep 9, 2018·Acta Biomaterialia·William J KelleyOmolola Eniola-Adefeso
Dec 7, 2005·Biochemical and Biophysical Research Communications·Nina TsaoChih-Feng Kuo

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