Abstract
Properdin (P)-deficient human serum containing type-specific anticapsular antibodies and having an intact classical pathway of complement did not support efficient granulocyte phagocytosis of Streptococcus pneumoniae serotypes 6A, 14, 19F, 23F, or 35 in an opsonophagocytic assay. Compared with pooled control serum, the difference was most pronounced for serotypes 14 and 23F and at a final serum concentration of 16%. The reduced phagocytic killing of S. pneumoniae serotype 23F in P-deficient serum was due rather to defective opsonization than to impaired intracellular killing. The uptake of C3 by the serotype 23F strain was found to be low in P-deficient serum. The addition of native P promoted C3 fixation as well as opsonization. The activity of P was abolished by heat treatment (56 degrees C, 30 min). Experiments with Mg EGTA to block C1 activation suggested that serotype 23F pneumococci were more dependent on the classical pathway for opsonization than were serotype 35 pneumococci, which appear to be partly opsonized through the alternative pathway alone.
References
Oct 1, 1975·Infection and Immunity·D P Fine
Aug 1, 1977·Infection and Immunity·C G StephensW P Reed
Nov 1, 1977·Infection and Immunity·G S GiebinkP G Quie
Feb 1, 1978·Immunochemistry·J O Minta, W Vetvutanapibul
Jan 1, 1978·Medicine·V Agnello
Apr 1, 1979·The Journal of Clinical Investigation·P C LeijhR van Furth
Dec 1, 1979·Infection and Immunity·H A VerbrughJ Verhoef
Aug 10, 1978·The New England Journal of Medicine·S L NewmanR B Johnston
Feb 1, 1977·The Journal of Experimental Medicine·A G Ehlenberger, V Nussenzweig
Jan 1, 1977·Journal of Immunological Methods·J VerhoefP G Quie
May 1, 1973·The Journal of Pediatrics·J A Winkelstein
Jan 1, 1966·International Archives of Allergy and Applied Immunology·P J McConahey, F J Dixon
Jan 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·P O Ganrot
Sep 29, 1966·The New England Journal of Medicine·F S Rosen, C A Janeway
Mar 1, 1982·Infection and Immunity·S E GardnerC J Baker
Jul 31, 1980·The New England Journal of Medicine·D T Fearon, K F Austen
Mar 1, 1982·Journal of Clinical Microbiology·F K Pedersen, J Henrichsen
Jan 1, 1982·The Journal of Clinical Investigation·E J BrownM M Frank
Jan 1, 1981·Infection and Immunity·K K MatthayA J Ammann
Citations
Jun 1, 1990·Antonie van Leeuwenhoek·J E van DamH Snippe
Oct 1, 1990·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A G Sjöholm
Jan 1, 1989·Scandinavian Journal of Infectious Diseases·C SöderströmS Ostenson
Jan 1, 1995·Scandinavian Journal of Infectious Diseases·K EkdahlJ H Braconier
Oct 10, 2007·Infection and Immunity·Jie LiDavid E Briles
Mar 1, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sunita GulatiSanjay Ram
Nov 1, 1991·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·C SöderströmB Thuresson
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Youssif Mohammed AliWilhelm J Schwaeble
Nov 13, 2008·Infection and Immunity·Jie LiDavid E Briles
Sep 9, 2018·Acta Biomaterialia·William J KelleyOmolola Eniola-Adefeso
Dec 7, 2005·Biochemical and Biophysical Research Communications·Nina TsaoChih-Feng Kuo