Granulocytic subset of myeloid derived suppressor cells in rats with mammary carcinoma

Cellular Immunology
Yusuf DolenDicle Guc

Abstract

Limited knowledge is available on myeloid derived suppressor cells (MDSCs) of rat origin. We examined the myeloid cells from peripheral blood, bone marrow and spleens of healthy and mammary tumor bearing rats employing a novel immunophenotyping strategy with CD172a, HIS48, and Rp-1 antibodies. We addressed rat granulocytes by Rp-1 positivity and used HIS48 in discrimination of two mononuclear cell subsets. An expansion of granulocyte numbers was detected in peripheral blood and spleens of mammary tumor-bearing animals. The purified granulocytes were able to impair antigen-specific helper T-cell proliferation, and therefore nominated as granulocytic MDSCs of this rat tumor model. HIS48(+) mononuclear cell numbers were also increased in the blood and spleens of mammary tumor bearing rats with a lower MHC class II positivity. Despite the lack of an antigen specific suppression of CD4(+) T cells, HIS48(+) monocytes resemble monocytic MDSCs with their inflammatory phenotype. Together, these results provide evidence for the existence and phenotypic characterization of a granulocytic MDSC subset in a rat model of mammary carcinoma.

References

Feb 15, 2000·International Reviews of Immunology·J T Attwood, D H Munn
Sep 29, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·D K YsebaertM E De Broe
Feb 7, 2009·Nature Reviews. Immunology·Dmitry I Gabrilovich, Srinivas Nagaraj
Jul 16, 2010·Blood·Loems Ziegler-HeitbrockManfred B Lutz
Oct 5, 2010·Scandinavian Journal of Immunology·T FahmiD Guc
Sep 29, 2011·Journal of Leukocyte Biology·Je-In YounDmitry I Gabrilovich
Apr 17, 2013·Cell Cycle·Je-In Youn, Dmitry I Gabrilovich

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Citations

Sep 4, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yaghoub YazdaniFarhad Jadidi-Niaragh
Aug 10, 2018·Environmental and Molecular Mutagenesis·Azra DadVasily N Dobrovolsky
Oct 9, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Wendy R FrancisIan Pallister

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