Granulopoiesis in long-term culture by marrow from mice with busulfan-induced chronic latent aplasia

International Journal of Cell Cloning
R L BoydA J Erslev

Abstract

Mice given high-dose busulfan therapy develop a chronic latent marrow aplasia characterized by normal peripheral blood neutrophil numbers, hematocrits and marrow cellularity but reduced numbers of pluripotent hemopoietic stem cells (CFU-s) and granulocyte-monocyte progenitor cells (CFU-gm). To study the pathogenesis of this lesion, bone marrow was propagated in long-term marrow cultures (LTMC). Small amounts of normal marrow readily established and sustained long-term granulopoiesis in vitro. In contrast, inocula of marrow from busulfan-treated animals containing three to five times as many stem and progenitor cells failed to establish long-term granulopoiesis in vitro. These results suggest that high-dose busulfan therapy produces a qualitative defect in either the hemopoietic stem cells, the stromal-forming elements, or both, rendering them incapable of establishing long-term granulopoiesis in vitro. Furthermore, mixing experiments employing normal and busulfan-damaged marrow demonstrate that this qualitative defect is not due to the emergence of a suppressor cell population. LTMC can show types of marrow damage not detectable by other techniques currently available and represent a powerful tool for studying latent bone marro...Continue Reading

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S HellmanR M Vigneulle
Jun 1, 1977·Journal of Cellular Physiology·T M DexterL G Lajtha
Apr 1, 1976·British Journal of Haematology·A MorleyJ Remes
Nov 1, 1985·International Journal of Cell Cloning·S A RothmannF Fantelli
Dec 1, 1974·The Australian Journal of Experimental Biology and Medical Science·A Morley, J Blake
Apr 1, 1969·Journal of Cellular Physiology·V K JenkinsT T Odell
Oct 1, 1982·Toxicology and Applied Pharmacology·R L BoydA J Erslev
Jan 1, 1981·Cell and Tissue Kinetics·J H FitchenM J Cline

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Citations

Jul 14, 2005·Clinical Medicine & Research·Jichun Chen
Sep 28, 2007·Environmental and Molecular Mutagenesis·Pedro Morales-Ramírez, Francisco González-Beltrán

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