Granulosa cell stimulation of thecal androgen synthesis

Canadian Journal of Physiology and Pharmacology
A Lischinsky, D T Armstrong

Abstract

The role of granulosa cells on porcine follicular androgen synthesis was studied. Granulosa cells were cultured for 24 h either in Eagle's minimum essential medium (MEM) or in MEM plus follicle-stimulating hormone (FSH; 1 microgram/mL). Thecal preparations were cultured with or without luteinizing hormone (LH), either in MEM, 50% "spent media" from granulosa cells control (MA) or 50% "spent media" from granulosa cells incubated with FSH (MB). In the absence of LH, MB stimulated accumulation of both delta 4-androstenedione (twofold) and testosterone (threefold). MB was found to contain high levels of C21-steroids, including progesterone and material which behaves chromatographically and immunologically like pregnenolone. These amounts of C21-steroids were able to stimulate thecal androgen production. The results suggest that granulosa cells contribute to thecal androgen production by providing steroid substrate. This would provide a means for granulosa cells to control the availability of aromatizable androgens.

Citations

Oct 1, 1983·Journal of Steroid Biochemistry·A LischinskyD T Armstrong
Jan 1, 1987·Journal of Steroid Biochemistry·S G Hillier
Jan 1, 1987·Journal of Steroid Biochemistry·G S DiZeregaG Westhof
Feb 21, 2008·The Journal of Clinical Endocrinology and Metabolism·Deborah S WachsR Jeffrey Chang
May 28, 2013·Fertility and Sterility·Yvonne D HoangShunichi Shimasaki
Jul 15, 2020·Reviews on Environmental Health·Konstantinos S KechagiasMaria Kyrgiou
Mar 29, 2019·Journal of the Endocrine Society·Michael V HomerR Jeffrey Chang
Nov 1, 1987·The American Journal of Physiology·P MorleyR E Gore-Langton
Mar 1, 1991·Molecular and Cellular Endocrinology·H EngelhardtD T Armstrong
Jun 1, 1988·Molecular and Cellular Endocrinology·R E Gore-Langton

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