Granulosa cells provide elimination of apoptotic oocytes through unconventional autophagy-assisted phagocytosis.

Human Reproduction
Marina G YefimovaCelia Ravel

Abstract

Do human granulosa cells (GCs) ingest and destroy apoptotic oocytes? Somatic GCs ingest and destroy apoptotic oocytes and other apoptotic substrates through unconventional autophagy-assisted phagocytosis. Most (99%) ovarian germ cells undergo apoptosis through follicular atresia. The mode of cleaning of atretic follicles from the ovary is unclear. Ovarian GCs share striking similarities with testicular Sertoli cells with respect to their origin and function. Somatic Sertoli cells are responsible for the elimination of apoptotic spermatogenic cells through unconventional autophagy-assisted phagocytosis. Human GCs were tested for the ability to ingest and destroy the apoptotic oocytes and other apoptotic substrates. A systemic study of the main phagocytosis steps has been performed at different time points after loading of apoptotic substrates into the GC. Primary cultures of GC retrieved following controlled ovarian stimulation of five women for IVF/ICSI and a human granulosa KGN cell line were incubated with different apoptotic substrates: oocytes which underwent spontaneous apoptosis during the cultivation of immature germ cells for IVF/ICSI; apoptotic KGN cells; and apoptotic membranes from rat retinas. Cultured GC were analy...Continue Reading

References

Jun 1, 1991·Cell and Tissue Research·C PineauB Jégou
Jun 1, 1969·The Journal of Experimental Medicine·R B JohnstonF S Rosen
May 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S A IgdouraL Hermo
Feb 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E BoschD Bok
Jan 1, 1996·Hormone Research·H KatabuchiH Okamura
Nov 25, 1997·Proceedings of the National Academy of Sciences of the United States of America·S C FinnemannE Rodriguez-Boulan
Feb 24, 1998·Biology of Reproduction·F GaytánJ E Sánchez-Criado
Jul 7, 1999·Journal of Reproductive Immunology·M ItohY Takeuchi
Feb 1, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·M A DavisN H Colburn
Nov 18, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·C M LaiP E Rakoczy
May 11, 2001·Nature·R S ScottG K Matsushima
Jan 31, 2003·Investigative Ophthalmology & Visual Science·Jacque L DuncanDouglas Vollrath
Mar 1, 1965·The Journal of Experimental Medicine·A C ALLISON, L MALLUCCI
Apr 10, 2004·Human Reproduction Update·Ruijin WuRebecca L Robker
May 13, 2005·Cellular Microbiology·Amal O Amer, Michele S Swanson
Mar 15, 2006·Trends in Cell Biology·Yi WuRaymond B Birge
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Dec 21, 2007·Biology of Reproduction·Marina G YefimovaFlorian Guillou
Feb 29, 2008·Nature·Noboru MizushimaDaniel J Klionsky
Apr 9, 2008·Critical Reviews in Oncology/hematology·Petr BenesMartin Fusek
Apr 19, 2008·Nature Reviews. Immunology·Greg Lemke, Carla V Rothlin
Jan 2, 2009·Investigative Ophthalmology & Visual Science·David J StrickDouglas Vollrath
Feb 7, 2009·The New England Journal of Medicine·Lawrence M Nelson
Feb 13, 2010·Fertility and Sterility·Jong Yeob ChoiDoo Seok Choi
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Chandrima ShahaDurga Prasad Mishra
Feb 23, 2011·Development, Growth & Differentiation·Yoshinobu NakanishiAkiko Shiratsuchi
Aug 2, 2011·Annual Review of Cell and Developmental Biology·Noboru MizushimaYoshinori Ohsumi
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jennifer MartinezDouglas R Green
Dec 16, 2011·Autophagy·Ellen WirawanPeter Vandenabeele
Jan 19, 2012·International Review of Cell and Molecular Biology·Nan Lu, Zheng Zhou
Mar 28, 2012·The Journal of Reproduction and Development·Fuko MatsudaSatoshi Ohkura
Feb 6, 2013·Autophagy·Irina TeplovaVassiliki Karantza
Feb 12, 2013·Advances in Experimental Medicine and Biology·Michelle K Y Siu, C Yan Cheng
Jul 23, 2013·Cell·Ji-Young KimThomas A Ferguson
Oct 18, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·JongYeob ChoiDooSeok Choi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms