Granulysin-mediated apoptosis of trophoblasts in blighted ovum and missed abortion

American Journal of Reproductive Immunology : AJRI
Tamara GulicDaniel Rukavina

Abstract

Granulysin (GNLY) is a cytotoxic molecule mostly present in decidual natural killer (NK) cells. Blighted ovum (BO) and missed abortion (MA) represent the early pathological pregnancies with hindered development of the embryoblast or a dead embryo. We investigated the GNLY-mediated apoptotic mechanism potentially responsible for delayed termination of pregnancy. We performed immunohistological and immunofluorescence labeling of decidual tissues (GNLY, Apaf-1, NF-κB). NKG2A expression was analyzed by flow cytometry and GNLY mRNA by RT-qPCR. The GNLY labeling intensity (H score) was lower in the nuclei of trophoblast cells in BO and MA. GNLY gene levels were inversely detected in BO and MA. A decreased decidual NK cell percentage was found in MA. NK cells from pathological pregnancies expressed lower NKG2A levels. The highest frequency of Apaf-1 was found in trophoblast cells of MA. NF-kB was highly expressed in decidual cells of BO. The reduced activation of GNLY-mediated killing might be implicated in the slower rejection of trophoblast cells in BO and MA. A decreased authentic decidual NK cell number could be responsible for low cytotoxicity against trophoblast cells in MA. In BO, trophoblast cells have a higher survival potent...Continue Reading

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Citations

Feb 22, 2021·The Journal of Obstetrics and Gynaecology Research·Ting LiuYan Fang
Mar 16, 2021·Frontiers in Cellular and Infection Microbiology·Teng LiXuemei Hu
Mar 16, 2021·Breast Care·Damir GrebićDanijela Veljković Vujaklija

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