Grape antioxidant dietary fibre reduced apoptosis and induced a pro-reducing shift in the glutathione redox state of the rat proximal colonic mucosa

The British Journal of Nutrition
María Elvira López-OlivaEmilia Muñoz-Martínez

Abstract

Grape antioxidant dietary fibre (GADF) is a grape product rich in dietary fibre and natural antioxidants. We showed previously that the GADF intake induced an epithelial hypoplasia in the rat colonic mucosa. In the present study, we propose that the antioxidant effect of GADF could modulate mucosal apoptosis via modulation of the cellular redox environment. Male Wistar rats (n 20) were fed with diets containing either cellulose (control diet group) or GADF (GADF diet group) as fibre for 4 weeks. The GSH:GSSG ratio, the redox state of the GSSG/2GSH couple (Ehc), the mitochondrial and/or cytosolic antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)), lipid peroxidation (LPO) and apoptosis were evaluated. GADF enhanced the cytosolic GSH:GSSG ratio, shifting the redox potential (Ehc) to a more pro-reducing status. Decreased Cu,ZnSOD:CAT, Cu,ZnSOD:GPx and MnSOD:GPx ratios could indicate an enhanced capacity for reducing H2O2, contributing to decreased cytosolic LPO. Reduced apoptosis in GADF-treated mucosa was inversely related to MnSOD activity. Furthermore, apoptosis increased directly as GSSG content increased. These results suggest that the reduction in apoptosis associated ...Continue Reading

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Citations

Aug 25, 2012·Journal of Oncology·Kequan Zhou, Julian J Raffoul
Dec 14, 2016·Journal of Agricultural and Food Chemistry·Raquel Del Pino-GarcíaPilar Muñiz
Jan 10, 2012·Journal of Food Science·María José PozueloIsabel Goñi
Aug 13, 2013·Nutrition Research Reviews·Jara Pérez-JiménezFulgencio Saura-Calixto
Mar 23, 2011·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Hua YaoZhuo Zhang
Oct 24, 2017·The British Journal of Nutrition·Emilce E LlopartSilvina R Drago
Aug 2, 2011·Journal of Agricultural and Food Chemistry·Wen-Tzu Wu, Hsiao-Ling Chen
Dec 15, 2010·Journal of Agricultural and Food Chemistry·Fulgencio Saura-Calixto

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis