Grapevine Grafting: Scion Transcript Profiling and Defense-Related Metabolites Induced by Rootstocks

Frontiers in Plant Science
Walter ChitarraGiorgio Gambino

Abstract

Rootstocks are among the main factors that influence grape development as well as fruit and wine composition. In this work, rootstock/scion interactions were studied using transcriptomic and metabolic approaches on leaves of the "Gaglioppo" variety, grafted onto 13 different rootstocks growing in the same vineyard. The whole leaf transcriptome of "Gaglioppo" grafted onto five selected rootstocks showed high variability in gene expression. In particular, significant modulation of transcripts linked to primary and secondary metabolism was observed. Interestingly, genes and metabolites involved in defense responses (e.g., stilbenes and defense genes) were strongly activated particularly in the GAG-41B combination, characterized in addition by the down-regulation of abscisic acid (ABA) metabolism. On the contrary, the leaves of "Gaglioppo" grafted onto 1103 Paulsen showed an opposite regulations of those transcripts and metabolites, together with the greater sensitivity to downy mildew in a preliminary in vitro assay. This study carried out an extensive transcriptomic analysis of rootstock effects on scion leaves, helping to unravel this complex interaction, and suggesting an interesting correlation among constitutive stilbenes, AB...Continue Reading

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Citations

Jul 29, 2018·Molecular Plant Pathology·Walter ChitarraGiorgio Gambino
Oct 6, 2018·Environmental Microbiology·L NervaM Turina
Mar 19, 2019·Frontiers in Plant Science·Guo-Qing SongVladimir Orbovic
Dec 24, 2019·Frontiers in Plant Science·Chiara PagliaraniGiorgio Gambino
Aug 22, 2020·Horticulture Research·Chiara PagliaraniAndrea Schubert
Jan 8, 2021·BMC Plant Biology·Massimiliano TrentiFrancesco Emanuelli
Apr 4, 2021·International Journal of Molecular Sciences·Giorgio GambinoIvana Gribaudo
Aug 2, 2021·Horticulture Research·Chiara PagliaraniAndrea Schubert

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Datasets Mentioned

BETA
GSE82317

Methods Mentioned

BETA
RNA-seq
PCR
transgenic

Software Mentioned

custom
IGGP
MapMan
DESeq
Bowtie2
Cufflinks
Primer Express®
SPSS
Excel
TopHat

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