Graphene oxide-assisted nucleic acids assays using conjugated polyelectrolytes-based fluorescent signal transduction

Analytical Chemistry
Fan LiLihua Wang

Abstract

In this work, we investigated the interactions between graphene oxide (GO) and conjugated polyelectrolytes (CPEs) with different backbone and side chain structures. By studying the mechanism of fluorescence quenching of CPEs by GO, we find that the charge and the molecular structure of CPEs play important roles for GO-CPEs interactions. Among them, electrostatic interaction, π-π interaction, and cation-π bonding are dominant driving forces. By using a cationic P2, we have developed a sensitive homogeneous sensor for DNA and RNA detection with a detection limit of 50 pM DNA and RNA, which increased the sensitivity by 40-fold as compared to GO-free CPE-based sensors. This GO-assisted CPE sensing strategy is also generic and shows a high potential for biosensor designs based on aptamers, proteins, peptides, and other biological probes.

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Citations

Dec 29, 2015·ACS Applied Materials & Interfaces·Yuanyuan ZhouYongqiang Cheng
Apr 18, 2017·ACS Applied Materials & Interfaces·Fan LiLihua Wang
Aug 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Haiyun LiuJinghua Yu
Mar 25, 2019·Analytical and Bioanalytical Chemistry·Juhong Chen, Sam R Nugen

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