Graphene oxide enhances alginate encapsulated cells viability and functionality while not affecting the foreign body response

Drug Delivery
Jesús CirizaJosé Luis Pedraz

Abstract

The combination of protein-coated graphene oxide (GO) and microencapsulation technology has moved a step forward in the challenge of improving long-term alginate encapsulated cell survival and sustainable therapeutic protein release, bringing closer its translation from bench to the clinic. Although this new approach in cell microencapsulation represents a great promise for long-term drug delivery, previous studies have been performed only with encapsulated murine C2C12 myoblasts genetically engineered to secrete murine erythropoietin (C2C12-EPO) within 160 µm diameter hybrid alginate protein-coated GO microcapsules implanted into syngeneic mice. Here, we show that encapsulated C2C12-EPO myoblasts survive longer and release more therapeutic protein by doubling the micron diameter of hybrid alginate-protein-coated GO microcapsules to 380 µm range. Encapsulated mesenchymal stem cells (MSC) genetically modified to secrete erythropoietin (D1-MSCs-EPO) within 380 µm-diameter hybrid alginate-protein-coated GO microcapsules confirmed this improvement in survival and sustained protein release in vitro. This improved behavior is reflected in the hematocrit increase of allogeneic mice implanted with both encapsulated cell types within 38...Continue Reading

References

Jan 5, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Buddy D Ratner
Feb 2, 2002·Experimental Hematology·Amelia BartholomewRonald Hoffman
Feb 4, 2005·Journal of Biomedicine & Biotechnology·Satya Prakash, Mitchell Lawrence Jones
Jun 7, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·G OriveJosé L Pedraz
Apr 25, 2006·Experimental Cell Research·Ida Rasmusson
Aug 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alma J NautaWillem E Fibbe
Oct 3, 2006·European Journal of Immunology·Antonio UccelliVito Pistoia
Oct 25, 2006·Xenotransplantation·Tsunehiro KobayashiGina R Rayat
Nov 16, 2006·Biomedical Microdevices·Shinji SugiuraMitsutoshi Nakajima
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tommy CedervallSara Linse
Nov 21, 2007·Advanced Drug Delivery Reviews·John T Wilson, Elliot L Chaikof
Dec 29, 2007·Seminars in Immunology·James M AndersonDavid T Chang
Apr 29, 2008·Biomaterials·David F Williams
Mar 18, 2009·Immunology and Cell Biology·Bart R TambuyzerPeter Ponsaerts
Apr 7, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gorka OriveJosé Luis Pedraz
Apr 21, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ainhoa MuruaJosé Luis Pedraz
Nov 21, 2009·Cytokine & Growth Factor Reviews·Lindolfo da Silva MeirellesArnold I Caplan
Apr 14, 2010·Advances in Experimental Medicine and Biology·Shinji Sakai, Koei Kawakami
Mar 1, 2011·Clinical and Experimental Immunology·M ShiF-S Wang
Nov 4, 2011·Journal of Microencapsulation·Micheal Whelehan, Ian W Marison
Nov 29, 2011·Trends in Molecular Medicine·Antje GeblerBarbara Seliger
Nov 30, 2011·Progress in Polymer Science·Kuen Yong Lee, David J Mooney
Sep 21, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Genaro A Paredes-JuarezPaul de Vos
Oct 29, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sumit GoenkaShilpa Sant
Feb 25, 2015·International Journal of Pharmaceutics·Haritz GurruchagaJose Luis Pedraz
Jul 30, 2015·International Journal of Pharmaceutics·J CirizaJ L Pedraz
Oct 9, 2015·Molecular Pharmaceutics·Ane GarateRosa Maria Hernandez
Jun 1, 2017·Molecular Pharmaceutics·Alberto Cañibano-HernándezJose Luis Pedraz

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Citations

Sep 27, 2019·Current Pharmaceutical Design·Romina MitarotondaMauricio C De Marzi
Aug 25, 2020·Advances in Pharmacological and Pharmaceutical Sciences·Dewi Melani Hariyadi, Nazrul Islam
May 21, 2020·Frontiers in Bioengineering and Biotechnology·Valentina PalmieriMassimiliano Papi
Jan 14, 2021·Carbohydrate Polymers·Pravin Shende, Nazneen Pathan
Mar 8, 2021·International Journal of Pharmaceutics·Kaoutar ZianiJose Luis Pedraz

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Methods Mentioned

BETA
xenograft
optical microscopy
ELISA
flow cytometry
Assay
flow
Fluorescence
Fluorescence microscopy

Software Mentioned

SPSS
Eclipse Net
[UNK]
Plex Manager
Bio
GraphPad Prism
Plex Pro

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