Graphene oxide immobilized enzymes show high thermal and solvent stability

Nanoscale
Soňa HermanováZdeněk Sofer

Abstract

The thermal and solvent tolerance of enzymes is highly important for their industrial use. We show here that the enzyme lipase from Rhizopus oryzae exhibits exceptionally high thermal stability and high solvent tolerance and even increased activity in acetone when immobilized onto a graphene oxide (GO) nanosupport prepared by Staudenmaier and Brodie methods. We studied various forms of immobilization of the enzyme: by physical adsorption, covalent attachment, and additional crosslinking. The activity recovery was shown to be dependent on the support type, enzyme loading and immobilization procedure. Covalently immobilized lipase showed significantly better resistance to heat inactivation (the activity recovery was 65% at 70 °C) in comparison with the soluble counterpart (the activity recovery was 65% at 40 °C). Physically adsorbed lipase achieved over 100% of the initial activity in a series of organic solvents. These findings, showing enhanced thermal stability and solvent tolerance of graphene oxide immobilized enzyme, will have a profound impact on practical industrial scale uses of enzymes for the conversion of lipids into fuels.

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Citations

Feb 6, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jing ChenAiping Xiao
Apr 6, 2017·Journal of Biomedical Materials Research. Part a·Milena RadunovicSusi Zara
May 24, 2017·Journal of Hazardous Materials·Hui LiVicki Chen
Mar 7, 2018·Preparative Biochemistry & Biotechnology·Shruthi SunderrajanGautam Pennathur
Oct 27, 2017·Chemistry : a European Journal·Jiri SturalaZdeněk Sofer
Aug 18, 2018·Chemistry : a European Journal·Soňa HermanováZdeněk Sofer
Jan 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhe DongFeng-Hong Huang
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Oct 20, 2018·ACS Applied Materials & Interfaces·Xiangming LiQi An

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