Graphene oxide surface blocking agents can increase the DNA biosensor sensitivity

Biotechnology Journal
Biwu LiuJuewen Liu

Abstract

Graphene oxide adsorbs single-strand fluorescent probe DNA, and the adsorbed probe can be desorbed by adding the complementary target DNA. Using this method, many biosensor studies have been carried out. We recently proposed a two-step mechanism for this sensing reaction: non-specific probe displacement followed by hybridization in the solution. Only about one out of six added target DNA is hybridized with the adsorbed probe to generate signal, leading to relatively low sensitivity. In this work, we aim to test whether surface blocking agents can minimize non-specific target adsorption and increase hybridization efficiency. Over ten blocking agents (polymers, surfactants, and DNA) were screened based on their effect on probe DNA adsorption and target DNA induced probe desorption. DNA oligonucleotides show significant and controllable enhancement in sensor sensitivity. The effect of DNA length and sequence was systematically investigated. Under optimized conditions, the sensor sensitivity was enhanced by nearly 10-fold. Using the same blocking method, sensitivity enhancement of other targets was also achieved, including adenosine and Hg(2+) with DNA aptamer probes. This reported surface blocking strategy can generally improve gr...Continue Reading

References

Nov 13, 2004·Angewandte Chemie·Eugenii Katz, Itamar Willner
Apr 14, 2005·Chemical Reviews·Nathaniel L Rosi, Chad A Mirkin
Jun 30, 2007·Angewandte Chemie·Itamar Willner, Maya Zayats
Sep 30, 2008·Chembiochem : a European Journal of Chemical Biology·Weian ZhaoYingfu Li
Mar 24, 2009·Chemical Reviews·Juewen LiuYi Lu
May 29, 2009·Angewandte Chemie·Chun-Hua LuGuo-Nan Chen
Sep 16, 2009·Accounts of Chemical Research·Xiaohong Fang, Weihong Tan
Feb 26, 2010·Analytical Chemistry·Haixin ChangJinghong Li
Mar 5, 2010·Nano Letters·Cristina Gómez-NavarroUte Kaiser
Mar 13, 2010·Accounts of Chemical Research·Di LiChunhai Fan
Jun 23, 2010·Journal of the American Chemical Society·Ying WangYuehe Lin
Sep 30, 2010·Angewandte Chemie·Chun-Hua LuGuo-Nan Chen
Feb 10, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Marissa WuJuewen Liu
Mar 8, 2012·Chemical Communications : Chem Comm·Min Zhang, Bang-Ce Ye
Apr 12, 2012·Analytical Chemistry·Po-Jung Jimmy Huang, Juewen Liu
Jun 29, 2012·Physical Chemistry Chemical Physics : PCCP·Juewen Liu
Aug 2, 2012·Journal of the American Chemical Society·Hao PeiChunhai Fan
Jul 24, 2013·Analytical Chemistry·Biwu LiuJuewen Liu
Jul 24, 2014·ACS Applied Materials & Interfaces·Qiao TangWeihong Tan
Oct 7, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Joon Soo ParkDong-Eun Kim
Jul 7, 2015·Chemical Society Reviews·Longhua TangJinghong Li

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Citations

Jun 9, 2016·Biotechnology Journal·Chunhai Fan, Eiichi Tamiya
Sep 27, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Chang LuJuewen Liu
Apr 19, 2017·Analytical Chemistry·Chen ShaoLeilei Tian
Feb 29, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Huan DuNansheng Cheng
Nov 26, 2020·Analytical and Bioanalytical Chemistry·Duy Ba NgoWerasak Surareungchai
Jan 29, 2021·Communications Materials·Shokoofeh SheibaniAdrian M Ionescu
Oct 5, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Sihui HeLeilei Tian
Nov 14, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Zhaoqi YangJian Jin

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