Graphene Quantum Dot-Based Nanocomposites for Diagnosing Cancer Biomarker APE1 in Living Cells

ACS Applied Materials & Interfaces
Hao ZhangFangwei Shao

Abstract

As an essential DNA repair enzyme, apurinic/apyrimidinic endonuclease 1 (APE1) is overexpressed in most human cancers and is identified as a cancer diagnostic and predictive biomarker for cancer risk assessment, diagnosis, prognosis, and prediction of treatment efficacy. Despite its importance in cancer, however, it is still a significant challenge nowadays to sense abundance variation and monitor enzymatic activity of this biomarker in living cells. Here, we report our construction of biocompatible functional nanocomposites, which are a combination of meticulously designed unimolecular DNA and fine-sized graphene quantum dots. Upon utilization of these nanocomposites as diagnostic probes, massive accumulation of fluorescence signal in living cells can be triggered by merely a small amount of cellular APE1 through repeated cycles of enzymatic catalysis. Most critically, our delicate structural designs assure that these graphene quantum dot-based nanocomposites are capable of sensing cancer biomarker APE1 in identical type of cells under different cell conditions and can be applied to multiple cancerous cells in a highly sensitive and specific manners. This work not only brings about new methods for cytology-based cancer screeni...Continue Reading

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Citations

Dec 20, 2020·Colloids and Surfaces. B, Biointerfaces·Tahereh Seifi, Ali Reza Kamali
May 10, 2021·International Journal of Biological Macromolecules·Parvaneh Ghaderi Shikhi-Abadi, Mohammad Irani
May 21, 2021·Nanomedicine : Nanotechnology, Biology, and Medicine·Elizabeth CampbellAnton V Naumov
Jan 16, 2021·ACS Biomaterials Science & Engineering·Rahul S Tade, Pravin O Patil
Sep 3, 2021·ACS Omega·Nattapon KuntipPrapasiri Pongprayoon

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