Graphene substrate for inducing neurite outgrowth

Biochemical and Biophysical Research Communications
Jeong Soon LeeJung Yul Lim

Abstract

A few recent studies demonstrated that graphene may have cytocompatibility with several cell types. However, when assessing cell behavior on graphene, there has been no precise control over the quality of graphene, number of graphene layers, and substrate surface coverage by graphene. In this study, using well-controlled monolayer graphene film substrates we tested the cytocompatibility of graphene for human neuroblastoma (SH-SY5Y) cell culture. A large-scale monolayer graphene film grown on Cu foils by chemical vapor deposition (CVD) could be successfully transferred onto glass substrates by wet transfer technique. We observed that graphene substrate could induce enhanced neurite outgrowth, both in neurite length and number, compared with control glass substrate. Interestingly, the positive stimulatory effect by graphene was achieved even in the absence of soluble neurogenic factor, retinoic acid (RA). Key genes relevant to cell neurogenesis, e.g., neurofilament light chain (NFL), were also upregulated on graphene. Inhibitor studies suggested that the graphene stimulation of cellular neurogenesis may be achieved through focal adhesion kinase (FAK) and p38 mitogen-activated protein kinase (MAPK) cascades. Our data indicate that...Continue Reading

References

Apr 29, 1996·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M A PriceR Treisman
Dec 13, 2006·Physical Review Letters·A C FerrariA K Geim
Jan 29, 2008·Biomaterials·Jung Yul LimHenry J Donahue
Mar 14, 2008·Current Medicinal Chemistry·M MilosoG Tredici
Oct 19, 2010·Cell Adhesion & Migration·Mariola R Chacón, Pietro Fazzari
Apr 22, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jung Yul LimHenry J Donahue
Mar 27, 2012·Theranostics·He ShenZhijun Zhang
Apr 11, 2012·International Journal of Molecular Sciences·Macarena PeránJuan A Marchal
Aug 29, 2012·Advanced Materials·Xiao HuangHua Zhang
Jun 7, 2013·BioResearch Open Access·Suzanne HigginsJung Yul Lim
Jun 13, 2013·Biomaterials·Mingliang TangGuosheng Cheng
Aug 3, 2013·Journal of Materials Science. Materials in Medicine·Meixian GuoHanqing Gu
Sep 24, 2013·Cellular Signalling·Susan DwanePatrick A Kiely
Aug 15, 2014·Bioconjugate Chemistry·Sixiang ShiWeibo Cai

❮ Previous
Next ❯

Citations

Feb 16, 2016·Biomaterials·Farida VelievCécile Delacour
Sep 24, 2016·ACS Applied Materials & Interfaces·Sachin Kumar, Kaushik Chatterjee
Feb 7, 2018·Frontiers in Neuroscience·Domenica ConvertinoCamilla Coletti
Jun 27, 2018·Advanced Healthcare Materials·Rachel A FischerRebecca M Sappington
Jan 31, 2019·Frontiers in Neuroscience·Niccolò Paolo PampaloniRossana Rauti
Aug 14, 2019·Advanced Healthcare Materials·Antoine BourrierCécile Delacour
Nov 17, 2020·Frontiers in Neuroscience·Domenica ConvertinoCamilla Coletti
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Gopal AgarwalAkshay Srivastava
Jun 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Neda Bahremandi TolouNicola Lisi
Nov 27, 2018·ACS Applied Materials & Interfaces·Chuchao ZhouZhenxing Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.