Abstract
A few recent studies demonstrated that graphene may have cytocompatibility with several cell types. However, when assessing cell behavior on graphene, there has been no precise control over the quality of graphene, number of graphene layers, and substrate surface coverage by graphene. In this study, using well-controlled monolayer graphene film substrates we tested the cytocompatibility of graphene for human neuroblastoma (SH-SY5Y) cell culture. A large-scale monolayer graphene film grown on Cu foils by chemical vapor deposition (CVD) could be successfully transferred onto glass substrates by wet transfer technique. We observed that graphene substrate could induce enhanced neurite outgrowth, both in neurite length and number, compared with control glass substrate. Interestingly, the positive stimulatory effect by graphene was achieved even in the absence of soluble neurogenic factor, retinoic acid (RA). Key genes relevant to cell neurogenesis, e.g., neurofilament light chain (NFL), were also upregulated on graphene. Inhibitor studies suggested that the graphene stimulation of cellular neurogenesis may be achieved through focal adhesion kinase (FAK) and p38 mitogen-activated protein kinase (MAPK) cascades. Our data indicate that...Continue Reading
References
Apr 29, 1996·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M A PriceR Treisman
Jan 9, 2004·Cell Cycle·Zhigang Xie, Li-Huei Tsai
Dec 13, 2006·Physical Review Letters·A C FerrariA K Geim
Jan 16, 2007·Biomaterials·Jung Yul LimHenry J Donahue
Jul 24, 2007·Biomaterials·Xiaomei LiuErwin A Vogler
Jan 29, 2008·Biomaterials·Jung Yul LimHenry J Donahue
Mar 14, 2008·Current Medicinal Chemistry·M MilosoG Tredici
Apr 5, 2008·Science·R R NairA K Geim
May 9, 2009·Science·Xuesong LiRodney S Ruoff
Oct 19, 2010·Cell Adhesion & Migration·Mariola R Chacón, Pietro Fazzari
Apr 22, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jung Yul LimHenry J Donahue
May 3, 2011·ACS Nano·Tapas R NayakBarbaros Özyilmaz
Sep 10, 2011·Biomaterials·Ning LiGuosheng Cheng
Oct 22, 2011·Biomaterials·G-Y ChenY-C Hu
Mar 27, 2012·Theranostics·He ShenZhijun Zhang
Apr 11, 2012·International Journal of Molecular Sciences·Macarena PeránJuan A Marchal
Aug 29, 2012·Advanced Materials·Xiao HuangHua Zhang
Sep 4, 2012·Acta Biomaterialia·Ishwari PoudelJung Yul Lim
Nov 20, 2012·Advanced Materials·Lingyan FengXiaogang Qu
May 28, 2013·Neuroscience·K J ChristieA M Turnley
Jun 7, 2013·BioResearch Open Access·Suzanne HigginsJung Yul Lim
Jun 13, 2013·Biomaterials·Mingliang TangGuosheng Cheng
Aug 3, 2013·Journal of Materials Science. Materials in Medicine·Meixian GuoHanqing Gu
Sep 24, 2013·Cellular Signalling·Susan DwanePatrick A Kiely
Aug 15, 2014·Bioconjugate Chemistry·Sixiang ShiWeibo Cai
Citations
Feb 16, 2016·Biomaterials·Farida VelievCécile Delacour
Nov 13, 2015·Biosensors & Bioelectronics·Jian HanQin Wei
Sep 24, 2016·ACS Applied Materials & Interfaces·Sachin Kumar, Kaushik Chatterjee
Feb 7, 2018·Frontiers in Neuroscience·Domenica ConvertinoCamilla Coletti
Jun 27, 2018·Advanced Healthcare Materials·Rachel A FischerRebecca M Sappington
May 12, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Chuan FuXiaoyu Yang
Aug 14, 2019·Journal of Biomedical Materials Research. Part a·Xianchun ChenChengjing Xue
Oct 19, 2017·Advanced Functional Materials·Anil KumarHong Liu
Jan 31, 2019·Frontiers in Neuroscience·Niccolò Paolo PampaloniRossana Rauti
Aug 14, 2019·Advanced Healthcare Materials·Antoine BourrierCécile Delacour
Dec 22, 2016·Frontiers in Bioengineering and Biotechnology·Çağla DefteralıCarlos Vicario-Abejón
Apr 27, 2018·Interface Focus·Arghya Narayan Banerjee
Jan 22, 2020·Neurotoxicity Research·Danuta JantasWładysław Lasoń
Nov 13, 2020·Neurochemical Research·Fangxuan ChuZhuo Yang
Nov 17, 2020·Frontiers in Neuroscience·Domenica ConvertinoCamilla Coletti
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Gopal AgarwalAkshay Srivastava
Apr 28, 2021·Inflammation and Regeneration·Sepehr AghajanianAlireza Shams
Jun 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Neda Bahremandi TolouNicola Lisi
Nov 27, 2018·ACS Applied Materials & Interfaces·Chuchao ZhouZhenxing Wang
Mar 27, 2020·Nano Letters·Domenica ConvertinoCamilla Coletti