Graphitic carbon nitride embedded hydrogels for enhanced gel electrophoresis

Analytica Chimica Acta
Mohammad ZareiAli Farzaneh

Abstract

Here, we show, for the first time, the use of graphitic carbon nitride (g-C3N4) nanosheets to improve the resolution and efficiency of protein separation in gel electrophoresis. By loading 0.04% (m/v) g-C3N4 nanosheets into the polyacrylamide gel at 25 °C, the thermal conductivity increased approximately 80% which resulted in 20% reduction in Joule heating and overall increase of separation efficiency. Also, polymerization of acrylamide occurred in the absence of tetramethylethylenediamine (TEMED) when the polyacrylamide gel contained g-C3N4 nanosheets. Hence, the g-C3N4 act simultaneously as a polymerization catalyst as well as heat sinks to lower Joule heating effect on band broadening.

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Citations

Feb 13, 2016·Chemical Society Reviews·Jian LiuMarkus Antonietti
Oct 15, 2018·Analytical and Bioanalytical Chemistry·Mohammad Zarei, Jamal Aalaie
Apr 28, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sayyed Hashem SajjadiHossein Ahmadzadeh

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