Abstract
Anti-cancer drugs generally kill cancer cells by apoptosis but fail to do so when they become resistant and escape apoptosis signals. But these resistant cells can still be killed by autophagy. Therefore, drugs having both apoptotic and autophagic abilities are solicited in effective cancer management. In search of such a drug, we examined the efficacy of graveoline, a bioactive compound isolated from Ruta graveolens on skin melanoma A375 cells through the use of specific signaling cascades and their inhibitors. Cytotoxicity of graveoline was tested by conducting MTT assay. Induction of autophagy and apoptosis was checked. Expression of related proteins and their localization were studied by conducting immunoblot assay and through confocal microscopy, respectively. We found graveoline-induced Beclin-1 associated autophagy in A375 cells and 3-methyladenine, an inhibitor of autophagy did not affect apoptosis. Conversely, caspase inhibitor that blocked apoptosis did not affect autophagic cell death, suggesting thereby that these two were independent events. Use of reactive oxygen species (ROS) scavengers inhibited cell death, but blocking autophagy did not affect graveoline-induced ROS generation, suggesting that ROS generation en...Continue Reading
References
Aug 1, 1972·British Journal of Cancer·J F KerrA R Currie
Aug 26, 1998·Trends in Cell Biology·D Green, G Kroemer
Nov 4, 2000·The EMBO Journal·Y KabeyaT Yoshimori
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·M Leist, M Jäättelä
Sep 6, 2001·Cell Death and Differentiation·W Bursch
Feb 6, 2003·Journal of Agricultural and Food Chemistry·Anna OlivaStephen O Duke
Apr 13, 2004·Oncogene·Devrim Gozuacik, Adi Kimchi
May 26, 2004·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Helene PelicanoPeng Huang
May 27, 2004·Journal of Agricultural and Food Chemistry·Amber L HaleStephen O Duke
Aug 25, 2004·The International Journal of Biochemistry & Cell Biology·Noboru Mizushima
Sep 24, 2005·Cell·Sophie PattingreBeth Levine
Oct 26, 2005·Cell Death and Differentiation·Y Tsujimoto, S Shimizu
Aug 3, 2006·Leukemia Research·Wenbin QianWeilai Xu
Sep 9, 2006·Cancer Cell·Paul T Schumacker
Feb 17, 2007·Dermatitis : Contact, Atopic, Occupational, Drug·Kimberly EickhorstJoan Csaposs
Jan 1, 2008·The Oncologist·Florence LefrancRobert Kiss
Jan 30, 2008·Autophagy·Yutaka MatsuiJunichi Sadoshima
May 24, 2008·Autophagy·Beth LevineGuido Kroemer
Jun 18, 2010·European Journal of Medicinal Chemistry·Zeng-Yun AnZhi-Shu Huang
Jun 8, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Raktim BiswasAnisur Rahman Khuda-Bukhsh
Oct 5, 2011·Critical Reviews in Eukaryotic Gene Expression·Che-Pei KungMaureen Murphy
Jan 31, 2012·Toxicology Letters·Debrup ChakrabortyAnisur Rahman Khuda-Bukhsh
Sep 4, 2012·European Journal of Pharmacology·Debrup ChakrabortyAnisur Rahman Khuda-Bukhsh
Mar 20, 2013·Journal of Integrative Medicine·Samrat GhoshAnisur Rahman Khuda-Bukhsh
Jul 1, 2008·Autophagy·Beth LevineGuido Kroemer
Citations
Jan 26, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zeng LiJuan Jin
May 8, 2015·Molecules and Cells·Kausik BishayeeSung-Oh Huh
Oct 30, 2016·Biochemical Pharmacology·Heather Graham Hambright, Rita Ghosh
Aug 29, 2017·Angewandte Chemie·Kang WangJianbo Wang
Jan 4, 2017·Oxidative Medicine and Cellular Longevity·Yanan NiuXiuping Chen
May 5, 2020·Current Drug Targets·Fatemeh Forouzanfar, Seyed Hadi Mousavi
Jun 14, 2020·Journal of Ethnopharmacology·Alexandra T CoimbraAna Paula Duarte
Feb 8, 2021·Mycopathologia·Laina Zarisa Mohd KamalDoblin Sandai
Jul 10, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Menglin LiuYanfei Li
Feb 6, 2020·Organic Letters·Shweta SinghGoverdhan Mehta