Gravimetric determination of the water concentration in whole blood, plasma and erythrocytes and correlations with hematological and clinicochemical parameters

Clinica Chimica Acta; International Journal of Clinical Chemistry
T H LijnemaC H Gips

Abstract

We have assessed gravimetric methods for determination of intravascular water, established whole blood-, plasma- and erythrocyte water reference values in a healthy volunteer group (n = 97, 48 females) and correlated these variables with 30 simultaneous hematological, clinicochemical and body parameters. The water standard was 55.56 mol/kg = 100 mass %. For erythrocyte water determination three methods were evaluated: 2 indirect methods were easy to perform, the third, using a hematocrit centrifuge, was the most reliable. Imprecision (within-batch coefficient of variation (CV), %) was excellent: whole blood 0.2, plasma 0.1, erythrocytes 0.7-2.2 and recoveries (means, %) 99.7-100.1. Serum water was found to be slightly higher than plasma water. Volunteer group, mean reference values, mass %: whole blood water 79.7, plasma water 91.2, erythrocyte water, three methods 66.2, 64.6 and 64.2, respectively. Females had mean 1.6 mass % higher whole blood water and 0.9-1.0 mass % higher erythrocyte water than males with no difference in plasma water. In the volunteer group whole blood water correlated strongly with hematocrit (r = -0.96), hemoglobin (r = -0.94) and erythrocytes (r = -0.85) and centrifuge hematocrit (r = -0.91). Plasma wa...Continue Reading

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Citations

Dec 22, 2019·Acta Anaesthesiologica Scandinavica·Robert G Hahn
Jul 17, 2004·Therapeutic Drug Monitoring·Alan Wayne Jones, Helena Larsson
Apr 27, 2020·BMC Anesthesiology·Robert G Hahn, Janis Nemme
Apr 4, 2021·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·William Quinn MeadusRichard B Thompson
Aug 6, 2021·Journal of Biomedical Materials Research. Part a·Ursula WindbergerLaurence Noirez

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