Gray-level co-occurrence matrix analysis of chromatin architecture in periportal and perivenous hepatocytes

Histochemistry and Cell Biology
Jovana PaunovicIgor Pantic

Abstract

Periportal hepatocytes (PPHs) and perivenous hepatocytes (PVHs) in standard optical microscopy appear to be morphologically identical. However, the functional properties of these two cell populations and their roles in liver lobules are not the same. Despite significant differences in gene expression between these two hepatocyte populations, it is still unclear whether the differences are present at the higher levels of chromatin organization. In this study, we present results, indicating that periportal and perivenous hepatocytes, when stained using toluidine blue histological dye, have different chromatin textural patterns quantified with gray-level co-occurrence matrix (GLCM) method. Hepatic tissue was obtained from ten male, healthy mice. Chromatin structures were analyzed using GLCM. For each structure, we measured the values of angular second moment, inverse difference moment, GLCM Contrast, GLCM Variance, and GLCM Sum Variance. The results indicate that there is a statistically significant difference in all GLCM mathematical parameters except the contrast. In addition, some chromatin GLCM features were in correlation with serum aminotransferase levels in perivenous, but not in periportal hepatocytes. To the best of our k...Continue Reading

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Citations

Dec 4, 2019·Ultrastructural Pathology·Hassan Reda Hassan ElsayedMostafa Mohammed Abd-Elmonem
Jun 27, 2020·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Marko DinčićAndjelija Ž Ilić
May 28, 2021·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Jovana PaunovicIgor Pantic
Dec 24, 2021·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Lazar M DavidovicIgor Pantic

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Methods Mentioned

BETA
biosensors

Software Mentioned

mathop
CellProfiler
GLCM

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