Great phenotypic and genetic variation among successive chronic Pseudomonas aeruginosa from a cystic fibrosis patient

PloS One
Carmen LozanoYolanda Sáenz

Abstract

Different adapted Pseudomonas aeruginosa morphotypes are found during chronic infections. Relevant biological determinants in P. aeruginosa successively isolated from a cystic fibrosis (CF) patient were analyzed in this work to gain insight into P. aeruginosa heterogeneity during chronic infection. Seventeen P. aeruginosa isolates collected from a patient over a 3 year period were included, 5 small colony variants (SCV) and 12 mucoids. The following analyses were performed: Pulsed-Field-Gel-Electrophoresis (PFGE)/Multilocus- sequence-typing (MLST)/serotype, antimicrobial susceptibility, growth curves, capacity to form biofilm, pigment production, elastase activity, motility; presence/expression of virulence/quorum sensing genes, and identification of resistance mechanisms. All isolates had closely related PFGE patterns and belonged to ST412. Important phenotypic and genotypic differences were found. SCVs were more resistant to antimicrobials than mucoid isolates. AmpC hyperproduction and efflux pump activity were detected. Seven isolates contained two integrons and nine isolates only one integron. All SCVs showed the same OprD profile, while three different profiles were identified among mucoids. No amino acid changes were foun...Continue Reading

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Citations

Jul 5, 2019·Science Translational Medicine·Sebastián A RiquelmeAlice Prince
Dec 15, 2020·Archives of Medical Research·Roberto Rosales-ReyesJosé Ignacio Santos-Preciado
Aug 28, 2021·International Journal of Molecular Sciences·Patrícia CoelhoLucinda J Bessa
Sep 10, 2021·PLoS Pathogens·Matthew J Culyba, Daria Van Tyne

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Datasets Mentioned

BETA
MF135190

Methods Mentioned

BETA
PCR
PCRs
Elastase assay

Software Mentioned

GraphPad Prism
GraphPad
commander
R
Image Lab
GelJ

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