PMID: 377284Apr 1, 1979

Greatly decreased susceptibility of nonmetabolizing cells towards detergents

Proceedings of the National Academy of Sciences of the United States of America
E KomorWidmar Tanner

Abstract

The addition of different detergents to Chlorella cells that had previously accumulated 6-deoxyglucose causes a rapid release of the hexose analogue into the medium. This effect is independent of the nature of the detergent and is observed only when the cells are in an energized state. Thus, in the presence of the uncoupler p-trifluoromethoxycarbonylcyanide phenylhydrazone or of inhibitors such as N-ethylmaleimide, the cells show a greatly reduced susceptibility towards detergents. Similarly, the detergent-induced loss of accumulated alpha-aminoisobutyric acid from Saccharomyces cerevisiae and of potassium from Escherichia coli is also strongly affected by the energy state of the cells. The differential susceptibility of energized and nonenergized cells was observed at all detergent concentrations tested. Measurements of substrate efflux at different concentrations of Triton indicated that only Triton monomers are responsible for the increase in permeability. The absorption of [14C]Triton X-100 by Chlorella and the binding of detergent to the cells were measured in the presence of metabolic inhibitors. Again, nonenergized cells bound a significantly lower amount of Triton X-100. The amphiphilic antibiotic nystatin produced effe...Continue Reading

References

Feb 1, 1977·Canadian Journal of Microbiology·V L OlsonD O McClary
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D Nieva-Gomez, R B Gennis
Mar 25, 1975·Biochimica Et Biophysica Acta·A Helenius, K Simons
Nov 2, 1972·Biochimica Et Biophysica Acta·A Kotyk, L Ríhová
Oct 1, 1972·Archives of Biochemistry and Biophysics·H Van ZutphenD G Cornwell
Jun 20, 1972·Biochimica Et Biophysica Acta·E KomorWidmar Tanner
Jan 1, 1966·The Journal of General Physiology·W Epstein, S G Schultz
Jul 23, 1969·Biochemical and Biophysical Research Communications·Widmar Tanner

Citations

Feb 23, 1994·Biochimica Et Biophysica Acta·A Ballarin-DentiH Kuroda
Jan 1, 1982·The International Journal of Biochemistry·M C BarberoF M Goñi
Jan 1, 1994·Progress in Biophysics and Molecular Biology·A G Sabelnikov
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D P DelmerE Padan
Sep 1, 1982·Plant Physiology·T M Murphy, C Wilson
Apr 1, 1987·Antimicrobial Agents and Chemotherapy·M HussainJ Lenard
Jul 8, 2009·Journal of Bacteriology·Maria João FriasMário Ramirez
May 4, 2013·Annual Review of Plant Biology·Jan MalinskyWidmar Tanner
Jul 31, 2012·Aquatic Biosystems·Devendra H DusaneSmita S Zinjarde
Aug 9, 2001·Biotechnology and Bioengineering·D G Brown, P R Jaffé
Nov 21, 2014·The FEBS Journal·Petr HermanJan Malinsky
Feb 24, 2016·Biochimica Et Biophysica Acta·Jan MalinskyMiroslava Opekarová
May 9, 2007·The Journal of Biological Chemistry·Enno C I VeermanArie V Nieuw Amerongen
Nov 4, 2020·FEBS Letters·Filipa C SantosRodrigo F M de Almeida

Related Concepts

Aminoisobutyric Acids
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
Chlorella
Deoxyglucose
Synthetic Detergent
Alkalescens-Dispar Group
Ethylmaleimide
Vigilon
Potassium
Saccharomyces cerevisiae

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