Green fluorescent protein as a convenient and versatile marker for studies on functional genomics in Drosophila

Development Genes and Evolution
Anja C NagelAnette Preiss

Abstract

Gene function can be deduced from lack or gain of activity. For the manipulation of gene doses or activity in Drosophila, a set of P-based vectors was constructed containing green fluorescent protein as marker. pBLUEi, pGREENi and pYELLi were designed for large insert transformation. Mosaicism was generated in vivo with pFlipG which is also ideal for targeted gene disruption. Tissue-specific gene silencing in vivo was performed with the vector set pHIBS and pUdsGFP. pHIBS allows easy cloning and shuttling of double-headed constructs. With pUdsGFP, double stranded RNA can be produced in defined patterns and the area of interference simultaneously visualized by green fluorescence. We demonstrate nearly complete silencing of a ubiquitously expressed gene in a tissue-specific manner.

Citations

Nov 24, 2004·Development Genes and Evolution·Volker Hartenstein, Diethard Tautz
Aug 19, 2003·Developmental Biology·Magali SuzanneErnesto Sánchez-Herrero
Jul 20, 2007·Molecular Biology of the Cell·Sabrina J Kugler, Anja C Nagel
Sep 12, 2006·Nucleic Acids Research·Marianne StabellAndrew Lambertsson
Jun 27, 2007·BMC Developmental Biology·Dieter MaierAnette Preiss
Jun 20, 2008·Genetics·Inma GonzálezAna Busturia
Jun 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Marc FurriolsJordi Casanova
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Apr 23, 2020·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Brindhi AmalrajEnrico Scarpella
Jan 25, 2008·Development·Marta Carrasco-Rando, Mar Ruiz-Gómez
Apr 6, 2007·Development·Joaquín de Navascués, Juan Modolell

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