PMID: 9447928Feb 3, 1998Paper

Green fluorescent protein as a reporter for gene transfer studies in the cochlea

Hearing Research
Anil K LalwaniAnand N Mhatre

Abstract

This study examined the 'humanized, red-shifted' version of the jellyfish Aequorea victoria green fluorescent protein (hrGFP) as a novel reporter for in vivo gene transfer studies in the cochlea using adeno-associated virus (AAV) vectors. Approximately 10(5) AAV vectors containing the hrGFP reporter gene were infused over 2 days or 1 week into the cochlea of the guinea pig via an osmotic minipump. Saline infused, non-infused, as well as AAV-beta-galactosidase infused guinea pigs served as the negative controls. The hrGFP transgene expression was detected as moderate intensity fluorescence easily distinguished from the background. Increased fluorescence was seen in the spiral ganglion, spiral ligament, spiral limbus, organ of Corti, and Reissner's membrane of the AAV-hrGFP infused animals. Control animals showed minimal fluorescence throughout the cochlea. Comparison of the 2 day and 1 week AAV-hrGFP infused animals showed qualitatively increased fluorescence in the 2 day animals. Background autofluorescence in the stria vascularis was noted in both the experimental and the control animals. In addition, fluorescence was detected in the contralateral cochlea of the AAV-hrGFP infused animals. Subsequent PCR analysis confirmed the ...Continue Reading

References

Jan 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J E Aubin
Jan 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C BensonG M McKhann
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G P NolanL A Herzenberg
Jun 1, 1971·Journal of Cellular Physiology·J G Morin, J W Hastings
Aug 31, 1981·Biochemical and Biophysical Research Communications·S H Bokman, W W Ward
Feb 23, 1995·Nature·R HeimR Y Tsien
Sep 5, 1994·FEBS Letters·S Inouye, F I Tsuji
Feb 11, 1994·Science·M ChalfieD C Prasher
Mar 29, 1996·Neuroscience Letters·Y RaphaelB J Roessler

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Citations

Oct 8, 1999·Hearing Research·T StöverY Raphael
Dec 31, 2003·Nuclear Medicine and Biology·Ronald G Blasberg
Oct 25, 2003·Progress in Retinal and Eye Research·Enrico M Surace, Alberto Auricchio
Jun 15, 2007·Gene Therapy·B W KesserJ R Holt
Aug 19, 2003·Ear and Hearing·Anil K Lalwani, Anand N Mhatre
Jun 8, 2000·Annals of the New York Academy of Sciences·T R Van de WaterH J Federoff
Nov 3, 2005·The European Journal of Neuroscience·Lisa N Gillespie, Robert K Shepherd
Jun 7, 2007·IEEE Transactions on Bio-medical Engineering·Lisa N PettingillRobert K Shepherd
Jun 25, 2013·Neurobiology of Aging·Wei Ping YangBo Hua Hu
Sep 27, 2008·Expert Opinion on Drug Delivery·Rachael T RichardsonStephen J O'Leary
May 15, 2001·Acta Oto-laryngologica·H StaeckerT R Van De Water
Jul 19, 2003·Neoplasia : an International Journal for Oncology Research·Vladimir PonomarevJuri Gelovani Tjuvajev
Feb 7, 2012·Autoimmunity Reviews·A GrecoM de Vincentiis
May 17, 2006·PLoS Biology·Grzegorz KudlaMaciej Zylicz
Mar 13, 2008·PloS One·Fiona RobinsonChristopher W J Smith
Nov 4, 2006·Hearing Research·Mark PraetoriusHinrich Staecker
Sep 20, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yuhe LiuKeiya Ozawa
Jun 1, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ida M StoneDavid J Poulsen
Dec 12, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·K KawamotoY Raphael
Oct 6, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·S T KhoA K Lalwani
Oct 12, 2004·Otolaryngologic Clinics of North America·Hinrich StaeckerKim Baker
Feb 28, 2008·The Laryngoscope·Bradley W KesserJeffrey R Holt
Sep 18, 2008·Current Opinion in Otolaryngology & Head and Neck Surgery·David A Borkholder
Nov 29, 2007·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Gentiana I WenzelJohn S Oghalai
May 14, 2005·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Farnoosh NoushiStephen O'Leary
Oct 9, 1998·The Journal of Laryngology and Otology·T J Bussoli, K P Steel
Nov 25, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kazuo OshimaYasufumi Kaneda
Mar 5, 2020·Laryngoscope Investigative Otolaryngology·Betsy SzetoAnil K Lalwani
Mar 20, 2020·Physiological Reviews·Sonja KleinlogelTobias Moser
Feb 20, 2021·Hearing, Balance and Communication·Chris ValentiniAnil K Lalwani
Mar 4, 2006·Brain Research·Mark A Crumling, Yehoash Raphael
Nov 23, 2006·Hematology/oncology Clinics of North America·Inna Serganova, Ronald G Blasberg

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