PMID: 9421525Feb 28, 1998Paper

Green fluorescent protein as a scaffold for intracellular presentation of peptides

Nucleic Acids Research
M R AbediA Kamb

Abstract

Peptide aptamers provide probes for biological processes and adjuncts for development of novel pharmaceutical molecules. Such aptamers are analogous to compounds derived from combinatorial chemical libraries which have specific binding or inhibitory activities. Much as it is generally difficult to determine the composition of combinatorial chemical libraries in a quantitative manner, determining the quality and characteristics of peptide libraries displayed in vivo is problematical. To help address these issues we have adapted green fluorescent protein (GFP) as a scaffold for display of conformationally constrained peptides. The GFP-peptide libraries permit analysis of library diversity and expression levels in cells and allow enrichment of the libraries for sequences with predetermined characteristics, such as high expression of correctly folded protein, by selection for high fluorescence.

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Citations

Dec 4, 2009·Plant Cell Reports·Nadine ParisGian Pietro Di Sansebastiano
Aug 2, 2003·Journal of Biotechnology·Giordano CaponigroAlexander Kamb
Oct 11, 2003·Biotechnology Advances·I Benhar
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Jul 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tej V PavoorEric V Shusta
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·G CaponigroA Kamb
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