Green fluorescent protein fusions to Arabidopsis fimbrin 1 for spatio-temporal imaging of F-actin dynamics in roots

Cell Motility and the Cytoskeleton
Yuh-Shuh WangElison B Blancaflor

Abstract

The visualization of green fluorescent protein (GFP) fusions with microtubule or actin filament (F-actin) binding proteins has provided new insights into the function of the cytoskeleton during plant development. For studies on actin, GFP fusions to talin have been the most generally used reporters. Although GFP-Talin has allowed in vivo F-actin imaging in a variety of plant cells, its utility in monitoring F-actin in stably transformed plants is limited particularly in developing roots where interesting actin dependent cell processes are occurring. In this study, we created a variety of GFP fusions to Arabidopsis Fimbrin 1 (AtFim1) to explore their utility for in vivo F-actin imaging in root cells and to better understand the actin binding properties of AtFim1 in living plant cells. Translational fusions of GFP to full-length AtFim1 or to some truncated variants of AtFim1 showed filamentous labeling in transient expression assays. One truncated fimbrin-GFP fusion was capable of labeling distinct filaments in stably transformed Arabidopsis roots. The filaments decorated by this construct were highly dynamic in growing root hairs and elongating root cells and were sensitive to actin disrupting drugs. Therefore, the fimbrin-GFP r...Continue Reading

References

May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·R O McCann, S W Craig
Mar 4, 1998·Plant Molecular Biology·D W McCurdy, M Kim
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Aug 3, 1999·European Journal of Cell Biology·H SawitzkyD Menzel
Dec 10, 1999·The Plant Cell·B C GibbonC J Staiger
Jun 10, 2000·Journal of Microscopy·A Geitmann, A M Emons
Nov 18, 2000·American Journal of Botany·E B Blancaflor, S Gilroy
Dec 21, 2000·The Plant Journal : for Cell and Molecular Biology·D R KovarD W McCurdy
Feb 22, 2001·Developmental Biology·F BaluskaD Volkmann
Jul 19, 2001·Genes & Development·E LemichezN H Chua
Dec 6, 2001·Protoplasma·D W McCurdyC J Staiger
Dec 24, 2002·Current Opinion in Plant Biology·Laurie G Smith
Jan 28, 2003·BioTechniques·Miki Nakazawa, Minami Matsui
May 3, 2003·Virology·Konduru KrishnamurthyJeanmarie Verchot-Lubicz
Jul 3, 2003·The Plant Cell·Jaideep MathurMartin Hülskamp
Aug 13, 2003·Plant Physiology·Shundai LiElizabeth M Lord
Sep 25, 2003·Annual Review of Plant Biology·Geoffrey O Wasteneys, Moira E Galway
Nov 13, 2003·Current Opinion in Plant Biology·David Ehrhardt
Jan 7, 2004·Nature Reviews. Molecular Cell Biology·Clive Lloyd, Jordi Chan

❮ Previous
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Citations

Dec 25, 2009·Sexual Plant Reproduction·Brian W Kram, Clay J Carter
Mar 30, 2006·Journal of Plant Research·Yoshihisa Oda, Seiichiro Hasezawa
Oct 3, 2008·Plant Molecular Biology·Brunilís Burgos-RiveraRichard B Meagher
Aug 25, 2011·Journal of Experimental Botany·Rosemary G White, Deborah A Barton
Dec 14, 2004·Plant Physiology·Geoffrey O Wasteneys, Zhenbiao Yang
May 4, 2006·Annual Review of Plant Biology·Patrick J HusseyMichael J Deeks
May 4, 2006·Annual Review of Plant Biology·Mark FrickerIan Moore
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Laurie G Smith, David G Oppenheimer
Mar 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Marci SurpinNatasha V Raikhel
Aug 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yuh-Shuh WangElison B Blancaflor
Jun 29, 2014·Journal of Plant Physiology·Ioannis-Dimosthenis S AdamakisEleftherios P Eleftheriou
Jul 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Antia Rodriguez-VillalonChristian S Hardtke
Dec 31, 2015·Proceedings of the National Academy of Sciences of the United States of America·David ScheuringJürgen Kleine-Vehn
Oct 26, 2010·Current Opinion in Plant Biology·Laurent BlanchoinChristopher J Staiger
Oct 16, 2007·Current Opinion in Plant Biology·Takumi HigakiSeiichiro Hasezawa
Nov 10, 2006·Trends in Plant Science·Jozef SamajDiedrik Menzel
Apr 26, 2005·Cell Motility and the Cytoskeleton·Luis CárdenasPeter K Hepler
Jun 9, 2009·Cell Motility and the Cytoskeleton·Clément ThomasAndré Steinmetz
Feb 13, 2008·The Plant Journal : for Cell and Molecular Biology·Raymond Wightman, Simon R Turner
Jan 31, 2006·The Plant Journal : for Cell and Molecular Biology·Ram DixitSimon Gilroy
Sep 30, 2006·Cell Motility and the Cytoskeleton·Carola L Holweg
Apr 11, 2007·The Plant Journal : for Cell and Molecular Biology·Abidur RahmanTobias I Baskin
Jun 30, 2010·Plant, Cell & Environment·Raul E Salinas-MondragonHeike Winter Sederoff
Mar 19, 2014·The Plant Journal : for Cell and Molecular Biology·Yanping FuZhensheng Kang
Sep 27, 2005·Current Opinion in Plant Biology·Alex Bannigan, Tobias I Baskin

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