Green process to prepare silk fibroin/gelatin biomaterial scaffolds
Abstract
A new all-aqueous and green process is described to form three-dimensional porous silk fibroin matrices with control of structural and morphological features. Silk-based scaffolds are prepared using lyophilization. Gelatin is added to the aqueous silk fibroin solution to change the silk fibroin conformation and silk fibroin-water interactions through adjusting the hydrophilic interactions in silk fibroin-gelatin-water systems to restrain the formation of separate sheet like structures in the material, resulting in a more homogenous structure. Water annealing is used to generate insolubility in the silk fibroin-gelatin scaffold system, thereby avoiding the use of organic solvents such as methanol to lock in the beta-sheet structure. The adjusting of the concentration of gelatin, as well as the concentration of silk fibroin, leads to control of morphological and functional properties of the scaffolds. The scaffolds were homogeneous in terms of interconnected pores, with pore sizes ranging from 100 to 600 microm, depending on the concentration of silk fibroin used in the process. At the same time, the morphology of the scaffolds changed from lamellar sheets to porous structures based on the increase in gelatin content. Compared wi...Continue Reading
References
Incorporation of growth factor containing Matrigel promotes vascularization of porous PLGA scaffolds
Citations
Synthetic adipose tissue models for studying mammary gland development and breast tissue engineering
Genipin-crosslinked gelatin-silk fibroin hydrogels for modulating the behaviour of pluripotent cells
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