Green process to prepare silk fibroin/gelatin biomaterial scaffolds

Macromolecular Bioscience
Qiang LuDavid L Kaplan

Abstract

A new all-aqueous and green process is described to form three-dimensional porous silk fibroin matrices with control of structural and morphological features. Silk-based scaffolds are prepared using lyophilization. Gelatin is added to the aqueous silk fibroin solution to change the silk fibroin conformation and silk fibroin-water interactions through adjusting the hydrophilic interactions in silk fibroin-gelatin-water systems to restrain the formation of separate sheet like structures in the material, resulting in a more homogenous structure. Water annealing is used to generate insolubility in the silk fibroin-gelatin scaffold system, thereby avoiding the use of organic solvents such as methanol to lock in the beta-sheet structure. The adjusting of the concentration of gelatin, as well as the concentration of silk fibroin, leads to control of morphological and functional properties of the scaffolds. The scaffolds were homogeneous in terms of interconnected pores, with pore sizes ranging from 100 to 600 microm, depending on the concentration of silk fibroin used in the process. At the same time, the morphology of the scaffolds changed from lamellar sheets to porous structures based on the increase in gelatin content. Compared wi...Continue Reading

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Citations

Sep 14, 2010·Journal of Mammary Gland Biology and Neoplasia·Xiuli WangDavid L Kaplan
May 29, 2012·Biomacromolecules·Cencen ZhangHesun Zhu
Sep 29, 2012·Biomacromolecules·Danyu YaoHesun Zhu
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Aug 3, 2010·Biomaterials·Xiao HuDavid L Kaplan
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May 4, 2016·ACS Applied Materials & Interfaces·Tingting LuoDavid L Kaplan
Jun 12, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Maria Christine Tankeh AsuncionSiew-Lok Toh
Jun 21, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shasha LinHesun Zhu
Nov 12, 2014·Advanced Functional Materials·Min D Tang-SchomerDavid L Kaplan
Jun 27, 2018·Journal of Biomaterials Science. Polymer Edition·Wenqian XiaoXiaoling Liao
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Mar 17, 2015·Advanced Healthcare Materials·Gang LiDavid L Kaplan
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Oct 9, 2015·Experimental Biology and Medicine·Danyu YaoYubo Fan
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Oct 8, 2020·BMJ Open Ophthalmology·Peter W MaddenMark J Ahearne
Dec 29, 2020·Frontiers in Bioengineering and Biotechnology·Li-Min YuZhi-Hai Fan
Jul 15, 2021·ACS Biomaterials Science & Engineering·Megan K DeBariRosalyn D Abbott
Aug 8, 2016·ACS Biomaterials Science & Engineering·Maria TorculasXiao Hu
Apr 11, 2016·ACS Biomaterials Science & Engineering·Kristin SchachtThomas Scheibel

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