Green Synthesis of AgNPs Stabilized with biowaste and their antimicrobial activities

Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]
Nakuleshwar Dut JasujaSuresh C Joshi

Abstract

In the present study, rapid reduction and stabilization of Ag+ ions with different NaOH molar concentration (0.5 mM, 1.0 mM and 1.5 mM) has been carried out in the aqueous solution of silver nitrate by the bio waste peel extract of P.granatum. Generally, chemical methods used for the synthesis of AgNPs are quite toxic, flammable and have adverse effect in medical application but green synthesis is a better option due to eco-friendliness, non-toxicity and safe for human. Stable AgNPs were synthesized by treating 90 mL aqueous solution of 2 mM AgNO₃ with the 5 mL plant peels extract (0.4% w/v) at different NaOH concentration (5 mL). The synthesized AgNPs were characterized by UV-Vis spectroscopy, TEM and SEM. Further, antimicrobial activities of AgNPs were performed on Gram positive i.e. Staphylococcus aureus, Bacillus subtilius and Gram negative i.e. E. coli, Pseudomonas aeruginosa bacteria. The AgNPs synthesized at 1.5 mM NaOH concentration had shown maximum zone of inhibition (ZOI) i.e. 49 ± 0.64 in E. coli, whereas Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilius had shown 40 ± 0.29 mm, 28 ± 0.13 and 42 ± 0.49 mm ZOI respectively. The MIC value of 30 μg/mL observed for E. coli Whereas, Staphylococcus aureu...Continue Reading

Citations

Jun 21, 2018·The Analyst·Amir GhasemiMichael R Hamblin
Oct 29, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Roberto NisticòGiuliana Magnacca
Sep 16, 2017·Environmental Science and Pollution Research International·Kadarkarai MuruganGiovanni Benelli
Nov 25, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Davi de Lacerda CoriolanoIsabella Macário Ferro Cavalcanti
Mar 2, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Nesreen A FatthallahMohamed A Shenashen

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Methods Mentioned

BETA
Transmission electron microscopy
surface plasmon resonance

Software Mentioned

SPSS

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