Green synthesis of silver nanoparticles using bovine skin gelatin and its antibacterial effect on clinical bacterial isolates

IET Nanobiotechnology
Hosam I SalaheldinGamal E H Osman

Abstract

Nanoparticles are being increasingly used in day-to-day life. Therefore, concerns have been raised regarding their interactions with the surrounding environment. This study focused on a simple green method for synthesizing silver nanoparticles (Ag-NPs) in an autoclave at 15 psi (103 kPa) and 121°C. An aqueous solution of AgNO3 as a precursor of Ag-NPs and gelatin (type B) reducing and/or stabilizing (capping) agent were used. The effect of various AgNO3 concentrations of certain gelatin concentration and various gelatin concentrations at constant AgNO3 concentration, and autoclaving time, was studied. UV-Vis spectra ascribed that the presence of localized surface plasmon resonance (SPR) of the synthesized Ag-NPs. TEM images and the selected area of electron diffraction confirmed, the formation of Ag-NPs with a diameter of approximately 5 ±0.35 nm. Furthermore, FT-IR revealed that a gelatin polymer matrix stabilized the synthesized Ag-NPs. The Well diffusion assay was used to test the effect of Ag-NPs on six clinical bacterial isolates, where Gram positive bacteria were more susceptible to Ag-NPs than Gram negative bacteria. Therefore, Ag-NPs capped by gelatin have remarkable potential effect as an antibacterial agent, and they ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Dec 6, 2003·Biotechnology Progress·S Shiv ShankarMurali Sastry
Nov 4, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Simon YoungAntonios G Mikos
May 24, 2006·Carbohydrate Research·N VigneshwaranP V Varadarajan
May 20, 2008·Journal of Colloid and Interface Science·Nishima WangooC Raman Suri
Oct 22, 2011·International Journal of Molecular Sciences·Majid DarroudiMohammad Hakimi
Jul 31, 2012·Chemistry Central Journal·Kamyar ShameliYadollah Gharayebi
Aug 28, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·K H Mahmoud, M Abbo
Sep 3, 2013·Indian Journal of Microbiology·Hussein H AbulreeshAbdulrahman S A Assaeedi

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