Green Synthesized Zinc Oxide (ZnO) Nanoparticles Induce Oxidative Stress and DNA Damage in Lathyrus sativus L. Root Bioassay System

Antioxidants
Kamal K PandaBrahma B Panda

Abstract

Zinc oxide nanoparticles (ZnONP-GS) were synthesised from the precursor zinc acetate (Zn(CH₃COO)₂) through the green route using the milky latex from milk weed (Calotropis gigantea L. R. Br) by alkaline precipitation. Formation of the ZnONP-GS was monitored by UV-visible spectroscopy followed by characterization and confirmation by energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), and X-ray diffraction (XRD). Both the ZnONP-GS and the commercially available ZnONP-S (Sigma-Aldrich) and cationic Zn(2+) from Zn(CH₃COO)₂ were tested in a dose range of 0-100 mg·L(-1) for their potency (i) to induce oxidative stress as measured by the generation reactive oxygen species (ROS: O₂(•-), H₂O₂ and (•)OH), cell death, and lipid peroxidation; (ii) to modulate the activities of antioxidant enzymes: catalase (CAT), superoxide dismutase (SOD), guaiacol peroxidase (GPX), and ascorbate peroxidase (APX); and (iii) to cause DNA damage as determined by Comet assay in Lathyrus sativus L. root bioassay system. Antioxidants such as Tiron and dimethylthiourea significantly attenuated the ZnONP-induced oxidative and DNA damage, suggesting the involvement of ROS therein. Our study demonstrated that both ZnONP-GS and ZnONP...Continue Reading

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Citations

Feb 20, 2019·Journal of Biomolecular Structure & Dynamics·Tahere KondoriVáclav Eigner
Nov 16, 2019·Human & Experimental Toxicology·S Şahin Doğan, A Kocabaş
Aug 15, 2018·IET Nanobiotechnology·Tejaswi ThunuguntaLakshmana Reddy Dhoranapalli Chinnappa Reddy
Aug 24, 2021·Journal of Toxicology·Chukwuebuka EgbunaChinwe Gloria Ibeabuchi

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Methods Mentioned

BETA
surface plasmon resonance
X-ray
Infrared Spectroscopy

Software Mentioned

Kinetic Komet TM Imaging Software
Excel
MATLAB

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